| 禾谷镰刀菌-玉米互作根系分泌物组分的鉴定及其抑菌活性分析 |
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| 引用本文：王云,江林,杨倩,谢珊珊.禾谷镰刀菌-玉米互作根系分泌物组分的鉴定及其抑菌活性分析.植物保护学报,2024,51(1):219-227 |
| DOI：10.13802/j.cnki.zwbhxb.2024.2022113 |
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| 中文摘要:为明确禾谷镰刀菌Fusarium graminearum侵染对玉米根系分泌物的影响，利用液相色谱-质谱（liquid chromatograph-mass spectrometer，LC-MS）联用技术和非靶向代谢组学分析手段鉴定侵染样品中的差异组分，并对差异组分的抑菌效果进行分析。结果表明，禾谷镰刀菌侵染后，玉米根系分泌物中共有34种组分发生差异表达，主要包括有机杂环化合物、苯类和有机氧化合物等，其中尼氟酸、咖啡酸乙酯、4-甲基-3-硝基苯甲酸和乙酸香兰素酯这4种组分对禾谷镰刀菌具有明显的抑制效果，以尼氟酸的抑菌效果最好，浓度为100 μg/mL时其对禾谷镰刀菌产孢量和3 h孢子萌发的抑制率分别达到96.0%和87.0%。此外，乙酸香兰素酯能使禾谷镰刀菌菌丝产生大量活性氧；而尼氟酸和4-甲基-3-硝基苯甲酸不仅诱导禾谷镰刀菌孢子产生大量活性氧，还引起菌丝和孢子的细胞坏死。表明筛选出的这4种抑菌组分可作为防治玉米茎基腐病的潜在资源。 |
| 中文关键词:禾谷镰刀菌 玉米 根系分泌物 代谢组学 抑菌组分 尼氟酸 |
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| Identification of root exudate components during the Fusarium graminearum-maize interaction and their antifungal activity |
| Author Name | Affiliation | E-mail | | Wang Yun | National and Local Joint Engineering Laboratory of Crop Stress Tolerance Breeding and Disaster Reduction, College of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui Province, China | | | Jiang Lin | National and Local Joint Engineering Laboratory of Crop Stress Tolerance Breeding and Disaster Reduction, College of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui Province, China | | | Yang Qian | National and Local Joint Engineering Laboratory of Crop Stress Tolerance Breeding and Disaster Reduction, College of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui Province, China | | | Xie Shanshan | National and Local Joint Engineering Laboratory of Crop Stress Tolerance Breeding and Disaster Reduction, College of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui Province, China | xssflora871216@126.com |
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| Abstract:In order to clarify the effects of fungal pathogen Fusarium graminearum infection on the root exudates of maize, liquid chromatography-mass spectrometer (LC-MS) technology and non-targeted metabolomics were used to identify the differential components. The antifungal effects of these differential components were further analyzed. The results showed that a total of 34 root exudate components were differentially expressed in response to F. graminearum infection, mainly including organic heterocyclic compounds, benzene and organic oxygen compounds. Among them, four components containing niflunic acid, ethyl caffeate, 4-methyl-3-nitrobenzoic acid and vanillin acetate, exhibited significant inhibitory effects on F. graminearum. The niflunic acid exerted the strongest antifungal activity against F. graminearum. The inhibitory rate of 100 μg/mL niflunic acid on spore production and spore germination reached 96.0% and 87.0%, respectively. In addition, vanillin acetate treatment caused reactive oxygen species (ROS) accumulation in the mycelium of F. graminearum; niflunic acid and 4-methyl-3-nitrobenzoic acid treatments not only induced ROS accumulation in the spores of F. graminearum, but also caused cell death of mycelium and spores. Taken together, the four components have the potential to control maize stem rot. |
| keywords:Fusarium graminearum maize root exudate metabolomics antifungal component niflunic acid |
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