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吲哚诱导对茶树抗茶小绿叶蝉的影响

引用本文：吕晓彤,周孝森,邹佳婷,辛肇军,蔡晓明,罗学平,蔡红兵,边磊.吲哚诱导对茶树抗茶小绿叶蝉的影响.植物保护学报,2024,51(1):114-122

DOI：10.13802/j.cnki.zwbhxb.2024.2022101

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作者	单位	E-mail
吕晓彤	中国农业科学院茶叶研究所, 农业农村部特种经济动植物生物学与遗传育种重点实验室, 杭州 310008
周孝森	中国农业科学院茶叶研究所, 农业农村部特种经济动植物生物学与遗传育种重点实验室, 杭州 310008
邹佳婷	中国农业科学院茶叶研究所, 农业农村部特种经济动植物生物学与遗传育种重点实验室, 杭州 310008
辛肇军	中国农业科学院茶叶研究所, 农业农村部特种经济动植物生物学与遗传育种重点实验室, 杭州 310008
蔡晓明	中国农业科学院茶叶研究所, 农业农村部特种经济动植物生物学与遗传育种重点实验室, 杭州 310008
罗学平	宜宾职业技术学院, 四川宜宾 644003
蔡红兵	四川省茶业集团股份有限公司, 宜宾 644002
边磊	中国农业科学院茶叶研究所, 农业农村部特种经济动植物生物学与遗传育种重点实验室, 杭州 310008	bianlei@tricaas.com

中文摘要:为明确吲哚处理对茶树抗茶小绿叶蝉Empoasca onukii的影响，以龙井43为材料，使用吲哚诱导茶树作为处理，以未处理茶树作为对照，于室内测定茶小绿叶蝉对不同茶树的选择趋向，利用昆虫刺探电位图谱分析茶小绿叶蝉的取食行为，通过气相色谱-质谱联用仪分析吲哚处理对茶树挥发物的影响，并利用实时荧光定量PCR技术测定茶树防御相关基因的表达量。结果表明，在所有时间段茶小绿叶蝉成虫更趋向于取食对照组茶树，至48 h时对照组茶树上的成虫数量是处理组的2.45倍；茶小绿叶蝉3龄幼虫在吲哚处理组茶树上的非取食波（NP波）持续时间占总取食时间的72.15%，在对照组茶树上的非取食波（NP波）持续时间占比则为33.07%，且在对照组茶树上的主要取食波E2波的持续时间是处理组茶树上的3.58倍。相较于对照组茶树，吲哚处理组茶树释放出的挥发物总量显著增加，其中α-法尼烯可以趋避害虫并对天敌有吸引力，且吲哚处理组茶树的防御基因表达量显著升高。表明吲哚处理茶树后，茶小绿叶蝉对其的取食量下降，茶树的挥发物释放量增加且抗性防御基因表达量提高，增强了茶树对茶小绿叶蝉的抗性。

中文关键词:茶树茶小绿叶蝉吲哚诱导防御取食行为抗性挥发物

Effect of indole treatment on the resistance to tea tree tea leafhopper Empoasca onukii

Author Name	Affiliation	E-mail
Lü Xiaotong	Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, Zhejiang Province, China
Zhou Xiaosen	Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, Zhejiang Province, China
Zou Jiating	Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, Zhejiang Province, China
Xin Zhaojun	Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, Zhejiang Province, China
Cai Xiaoming	Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, Zhejiang Province, China
Luo Xueping	Yibin Vocational and Technical College, Yibin 644003, Sichuan Province, China
Cai Hongbing	Sichuan Tea Industry Group Co., Ltd., Yibin 644002, Sichuan Province, China
Bian Lei	Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, Zhejiang Province, China	bianlei@tricaas.com

Abstract:In order to clarify the effect of indole treatment on the resistance of tea tree to tea green leafhopper Empoasca onukii, the cultivar Longjing 43 was used for indole induction, and the selection of E. onukii for different tea trees was measured in the laboratory. The electrical penetration graph (EPG) technique was used to analyze the feeding behavior of E. onukii. The effects of indole treatment on the volatiles of tea trees were analyzed with gas chromatography-mass spectrometry, and the expression levels of defense-related genes were detected by real-time fluorescence quantitative PCR. The results showed that, during all periods, E. onukii adults tended to feed on tea trees in the control group, and the number of adults in the control group was 2.45 times of that in the indole-treated group. The duration of third instar nymph’ s non-feeding wave (NP wave) on indole-treated tea trees accounted for 72.15% of the total feeding time, and the duration of non-feeding wave (NP wave) in the control group accounted for 33.07%. The duration of main feeding wave E2 in the control group was 3.58 times of that in the indole-treated group. Compared with the control group, the total amount of volatiles released by indoletreated tea trees was significantly increased, among which α-farnesene can deter pests but attract natural enemies. Moreover, the expression level of defense genes in indole-treated tea trees was significantly increased. The results indicated that the tea trees treated with indoles decreased food intake, increased the amount of volatiles and the expression level of resistance genes, which help to enhance the resistance of tea tree to E. onukii.

keywords:tea tree Empoasca onukii indole induced defense feeding behavior resistance volatile

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