欢迎访问《植物保护学报》网站！

首页 | 期刊简介 |
编委会
编委会
青年编委会
| 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English

RNA干扰介导HvIAP5基因沉默对茄二十八星瓢虫存活和发育的影响

引用本文：周楚翘,阮雅琳,杨春晓,张友军,潘慧鹏.RNA干扰介导HvIAP5基因沉默对茄二十八星瓢虫存活和发育的影响.植物保护学报,2025,52(4):924-933

DOI：10.13802/j.cnki.zwbhxb.2025.2025024

摘要点击次数:

全文下载次数:

作者	单位	E-mail
周楚翘	华南农业大学植物保护学院, 绿色农药全国重点实验室, 生物防治教育部工程研究中心, 广州 510642
阮雅琳	华南农业大学植物保护学院, 绿色农药全国重点实验室, 生物防治教育部工程研究中心, 广州 510642
杨春晓	华南农业大学植物保护学院, 绿色农药全国重点实验室, 生物防治教育部工程研究中心, 广州 510642
张友军	中国农业科学院蔬菜花卉研究所, 蔬菜生物育种全国重点实验室, 北京 100081
潘慧鹏	华南农业大学植物保护学院, 绿色农药全国重点实验室, 生物防治教育部工程研究中心, 广州 510642	panhuipeng@scau.edu.cn

中文摘要:为评估凋亡抑制蛋白5（inhibitor of apoptosis protein 5，IAP5）编码基因作为高效防控靶标基因的潜力，以茄二十八星瓢虫Henosepilachna vigintioctopunctata为研究对象，系统测定HvIAP5基因在茄二十八星瓢虫中的时空表达模式及组织特异性，通过饲喂体外合成和菌液表达产生的dsHvIAP5探讨HvIAP5沉默对茄二十八星瓢虫的存活率、生长发育及细胞凋亡的影响。结果显示：在茄二十八星瓢虫各发育阶段和各组织中HvIAP5基因均有广泛表达，主要在4龄幼虫阶段和中肠组织中高表达，成虫和马氏管中相对表达量则较少。饲喂体外合成的dsHvIAP5后，茄二十八星瓢虫的存活率较对照显著降低，其中饲喂浓度为100、200和300 ng/μL的dsHvIAP5浸泡叶片2 d后，1龄幼虫的存活率分别为64.0%、30.0%和12.0%；单头饲喂100、200和500 ng的dsHvIAP5后，4龄幼虫的存活率分别为58.0%、32.0%和2.0%，且取食dsHvIAP5 200 ng后2 d，HvIAP5基因的表达量显著下降了90.8%。饲喂菌液表达产生的dsHvIAP5后，茄二十八星瓢虫1龄和4龄幼虫的存活率分别达到10.0%和4.0%。TUNEL染色结果显示摄食dsHvIAP5显著促进了茄二十八星瓢虫幼虫中肠组织的细胞凋亡。综上所述，HvIAP5是潜在的RNA干扰高效靶标基因。

中文关键词:茄二十八星瓢虫凋亡抑制蛋白饲喂法 RNA干扰细胞凋亡

RNA interference-mediated silencing of HvIAP5 impairs survival and development of the 28-spotted ladybeetle Henosepilachna vigintioctopunctata

Author Name	Affiliation	E-mail
Zhou Chuqiao	Engineering Research Center of Biological Control, Ministry of Education State Key Laboratory of Green Pesticide College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Ruan Yalin	Engineering Research Center of Biological Control, Ministry of Education State Key Laboratory of Green Pesticide College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Yang Chunxiao	Engineering Research Center of Biological Control, Ministry of Education State Key Laboratory of Green Pesticide College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Zhang Youjun	State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Pan Huipeng	Engineering Research Center of Biological Control, Ministry of Education State Key Laboratory of Green Pesticide College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China	panhuipeng@scau.edu.cn

Abstract:To evaluate the potential of the inhibitor of apoptosis protein 5 (HvIAP5) as an efficient RNA interference (RNAi) target gene for pest management, its temporal and spatial expression patterns and tissue specificity in 28-spotted ladybeetle Henosepilachna vigintioctopunctata were investigated. The effects of HvIAP5 silencing on survival, development and apoptosis were evaluated using doublestranded RNA (dsHvIAP5) synthesized in vitro and expressed in bacteria. HvIAP5 was broadly expressed across all developmental stages and tissues, with the highest expression observed in the 4thinstar larvae and midgut, and lower levels in adults and Malpighian tubules. Feeding in vitro-synthesized dsHvIAP5 significantly reduced survival: after two days of exposure to 100, 200, and 300 ng/μL dsHvIAP5, survival rates 1st-instar larvae were 64.0%, 30.0% and 12.0%, respectively; 4th-instar larvae receiving 100, 200, and 500 ng per individual showed survival rates of 58.0%, 32.0%, and 2.0%, respectively, with a 90.8% reduction in HvIAP5 expression after two days of fed with 200 ng dsHvIAP5. Feeding bacterial-expressed dsHvIAP5 resulted in survival rates of 10.0% and 4.0% for 1st- and 4th-instar larvae, respectively. TUNEL staining indicated that dsHvIAP5 feeding significantly promoted apoptosis in midgut tissues.These findings suggest that HvIAP5 is a promising RNAi target gene for the control of H. vigintioctopunctata.

keywords:Henosepilachna vigintioctopunctata inhibitor of apoptosis protein feeding assay RNA interference apoptosis

查看全文查看/发表评论下载PDF阅读器