欢迎访问《植物保护学报》网站！

首页 | 期刊简介 |
编委会
编委会
青年编委会
| 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English

累西菲北里孢菌GD3-16的分离鉴定及其对香蕉枯萎病的防效

引用本文：田煜瑞,朱杰,陆月媚,蔡雨霏,李华平,李云锋,聂燕芳.累西菲北里孢菌GD3-16的分离鉴定及其对香蕉枯萎病的防效.植物保护学报,2025,52(4):859-869

DOI：10.13802/j.cnki.zwbhxb.2025.2025056

摘要点击次数:

全文下载次数:

作者	单位	E-mail
田煜瑞	华南农业大学植物保护学院, 广州 510642 华南农业大学广东省微生物信号与作物病害重点实验室, 广州 510642
朱杰	华南农业大学植物保护学院, 广州 510642 华南农业大学广东省微生物信号与作物病害重点实验室, 广州 510642
陆月媚	华南农业大学植物保护学院, 广州 510642 华南农业大学广东省微生物信号与作物病害重点实验室, 广州 510642
蔡雨霏	华南农业大学植物保护学院, 广州 510642 华南农业大学广东省微生物信号与作物病害重点实验室, 广州 510642
李华平	华南农业大学植物保护学院, 广州 510642 华南农业大学广东省微生物信号与作物病害重点实验室, 广州 510642
李云锋	华南农业大学植物保护学院, 广州 510642 华南农业大学广东省微生物信号与作物病害重点实验室, 广州 510642	yunfengli@scau.edu.cn
聂燕芳	华南农业大学广东省微生物信号与作物病害重点实验室, 广州 510642 华南农业大学材料与能源学院, 广州 510642	yanfangnie@scau.edu.cn

中文摘要:为获得对香蕉枯萎病有良好防效的生防菌，采用平板对峙培养法从健康香蕉根际土壤中分离菌株，以尖孢镰刀菌古巴专化型4号小种（Fusarium oxysporum f.sp.cubense race 4，Foc4）为指示菌筛选拮抗菌株，根据菌落形态、生理生化特征和多基因序列分析等对其进行鉴定，并测定拮抗菌株的抑菌谱、盆栽防效和对香蕉植株的促生作用，分析其促生潜力。结果显示：从巴西蕉根际土壤分离纯化共获得102株细菌，其中菌株GD3-16对Foc4的抑制效果最好，抑制率为62.8%。根据菌落形态、生理生化特征和多基因序列分析等将菌株GD3-16鉴定为累西菲北里孢菌Kitasatospora recifensis。该菌株可导致Foc4菌丝膨大畸形，对分生孢子萌发的抑制率为43.9%。菌株GD3-16产生的挥发性物质对Foc4菌丝生长抑制率高达91.6%，经鉴定其挥发性物质包含13种化合物。菌株GD3-16还具有广谱的抑菌活性，对10种植物病原真菌均有较好的抑制效果。菌株GD3-16对香蕉枯萎病的防效可达59.6%，对香蕉植株有显著的促生作用，还具有降解木质素、产生蛋白酶和铁载体的能力。表明菌株GD3-16有良好的开发和应用潜力。

中文关键词:香蕉枯萎病尖孢镰刀菌古巴专化型累西菲北里孢菌生物防治促生作用防效

Isolation and identification of bacterium Kitasatospora recifensis strain GD3-16 and its control effect against banana Fusarium wilt

Author Name	Affiliation	E-mail
Tian Yurui	College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Zhu Jie	College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Lu Yuemei	College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Cai Yufei	College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Li Huaping	College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, Guangdong Province, China
Li Yunfeng	College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong Province, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, Guangdong Province, China	yunfengli@scau.edu.cn
Nie Yanfang	Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, Guangdong Province, China College of Materials and Energy, South China Agricultural University, Guangzhou 510642, Guangdong Province, China	yanfangnie@scau.edu.cn

Abstract:To obtain biocontrol bacteria for the control of banana Fusarium wilt disease, antagonistic bacteria with good inhibitory effects on Fusarium oxysporum f. sp. cubense race 4 (Foc4) were isolated from the healthy banana rhizosphere soil using plate confrontational culture method. The antagonistic strain was identified by a combination of morphological observation, physiological and biochemical characteristics, and phylogenetic analysis of multiple genes including 16S rDNA, TrpB, RpoB, and GyrB. The anti-fungal spectrum, biological control and growth-promoting efficiency of the antagonistic strain were also investigated. The results showed that a total of 102 bacterial strains were isolated from the rhizosphere soil of banana plants. The strain GD3-16 had the highest antagonistic activity against Foc4 with an inhibition rate of 62.8%, which was further identified to be Kitasatospora recifensis with colony morphology, physiological and biochemical characteristics, and phylogenetic analysis of multiple genes. The strain GD3-16 altered Foc4 hyphal morphology by causing malformation and enlargement. The strain GD3-16 also inhibited spore germination of Foc4 significantly with an inhibition rate of 43.9%. Volatile organic compounds (VOCs) from the strain GD3-16 significantly inhibited fungal mycelial growth with an inhibition rate of up to 91.6% using the dual plate assay. Thirteen VOCs were identified through gas chromatography-mass spectrometry. The strain GD3-16 exhibited broadspectrum antagonistic activity to ten phytopathogenic fungi tested. Furthermore, K. recifensis GD3-16 had significant control effects on banana Fusarium wilt with a biocontrol efficiency of 59.6%. The strain GD3-16 effectively promoted banana growth, which possessed abilities to degrade lignin, secrete proteases and produce siderophores. In conclusion, K. recifensis GD3-16 has the potential as the ideal biocontrol agent in controlling banana Fusarium wilt with high efficiency and plant growth-promoting properties.

keywords:banana Fusarium wilt Fusarium oxysporum f. sp. cubense Kitasatospora recifensis biological control plant growth-promoting control effect

查看全文查看/发表评论下载PDF阅读器