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甲基二磺隆胁迫下抗、感小麦品种的生理响应差异

引用本文：侯燕茹,赵杰,高兴祥,赵铂锤,郭兵博,许贤,李秉华,谷旭,魏帅坤,王贵启,张颖君,刘小民.甲基二磺隆胁迫下抗、感小麦品种的生理响应差异.植物保护学报,2025,52(4):832-841

DOI：10.13802/j.cnki.zwbhxb.2025.2025008

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作者	单位	E-mail
侯燕茹	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035
赵杰	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035
高兴祥	山东省农业科学院植物保护研究所, 山东省农业有害生物绿色防控重点实验室, 济南 250100
赵铂锤	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035
郭兵博	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035
许贤	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035
李秉华	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035
谷旭	石家庄市藁城区植物保护检疫站, 石家庄 052160
魏帅坤	河北北方学院农林科技学院, 张家口 075000
王贵启	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035
张颖君	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035	zhangyingjun1977@163.com
刘小民	河北省农林科学院粮油作物研究所, 河北省作物栽培生理与绿色生产重点实验室, 石家庄 050035	liuxiaominsjz@gmail.com

中文摘要:为明确小麦对甲基二磺隆抗性差异的生理机制，测定甲基二磺隆对抗药小麦品种长4640（C-4640）和敏感小麦品种河农4198（H-4198）幼苗生长的影响，并测定15.75 g（a.i.）/hm²（田间最大推荐剂量）甲基二磺隆胁迫下两种小麦品种的抗氧化酶活性、丙二醛（malondialdehyde，MDA）含量、叶绿素含量和光合特征参数的响应差异，进一步采用相关性分析和主成分分析法确定小麦抗药性评价的关键指标。结果显示：甲基二磺隆对C-4640和H-4198的EC₅₀分别为63.20 g（a.i.）/hm²和4.22 g（a.i.）/hm²，前者是后者的15倍左右。15.75 g（a.i.）/hm²甲基二磺隆胁迫下，C-4640和H-4198体内超氧化物歧化酶、过氧化物酶和过氧化氢酶活性均呈上升趋势，并在施药后第5天达到峰值，且C-4640的抗氧化酶活性普遍高于H-4198；15.75 g（a.i.）/hm²甲基二磺隆胁迫下，C-4640体内MDA含量呈先升高后下降趋势，而H-4198体内MDA含量在第5天较对照下降28.62%，之后开始持续上升；H-4198体内叶绿体色素含量较对照显著降低，而气孔导度、蒸腾速率和净光合速率等光合特征参数较对照降低，而C-4640较对照无显著变化。表明叶绿体色素含量、光合特征参数及抗氧化酶活性可作为评价小麦对甲基二磺隆抗性的关键指标。

中文关键词:小麦甲基二磺隆生理响应抗氧化酶活性光合参数

Differences in physiological responses of resistant and susceptible wheat varieties to mesosulfuron-methyl stress

Author Name	Affiliation	E-mail
Hou Yanru	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China
Zhao Jie	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China
Gao Xingxiang	Shandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Zhao Bochui	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China
Guo Bingbo	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China
Xu Xian	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China
Li Binghua	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China
Gu Xu	Gaocheng Plant Protection and Quarantine Station, Shijiazhuang 052160, Hebei Province, China
Wei Shuaikun	College of Agriculture and Forestry Technology, Hebei North University, Zhangjiakou 075000, Hebei Province, China
Wang Guiqi	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China
Zhang Yingjun	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China	zhangyingjun1977@163.com
Liu Xiaomin	Key Laboratory of Crop Cultivation Physiology and Green Production in Hebei Province, Institute of Creal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei Province, China	liuxiaominsjz@gmail.com

Abstract:To examine the physiological mechanisms that underlie the observed variations in resistance to herbicide metildisulfuron among diverse wheat varieties, the effects of mesosulfuron-methyl on the growth of seedlings of the resistant wheat variety Chang 4640 (C-4640) and the susceptible variety Henong 4198 (H-4198) were determined, along with the response differences in antioxidant enzyme activities, malondialdehyde (MDA) content, chlorophyll content, and photosynthetic characteristic parameters under 15.75 g (a. i.)/hm²mesosulfuron-methyl stress (the maximum recommended field dose). Correlation analysis and principal component analysis (PCA) were further employed to identify key indicators for evaluating herbicide resistance in wheat varieties. The study showed that the EC₅₀ values of mesosulfuron-methyl for C-4640 and H-4198 were 63.20 g (a.i.)/hm²and 4.22 g (a.i.)/hm², respectively, with the former being approximately 15 times higher than the latter. Under 15.75 g (a.i.)/hm²mesosulfuron-methyl stress, the activities of superoxide dismutase, peroxidase, and catalase in both C-4640 and H-4198 increased, peaking on day 5 after treatment, and the antioxidant enzyme activities were generally higher in C-4640 than in H-4198; MDA content in C-4640 showed an initial increase followed by a decrease, while in H-4198, it decreased by 28.62% relative to the control on day 5 before rising continuously; chlorophyll content in H-4198 decreased significantly compared with the control. However, a decline in photosynthetic parameters, including stomatal conductance, transpiration rate, and net photosynthetic rate, was observed in the experimental group when compared with the control group. In contrast, C-4640 exhibited no statistically significant alterations in these parameters. The evaluation of wheat resistance to mesosulfuron-methyl can be facilitated by the use of key indicators, including chloroplast pigment content, photosynthetic characteristics, and antioxidant enzyme activity.

keywords:wheat mesosulfuron-methyl physiological response antioxidant enzyme activity photosynthetic parameter

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