| 不结球白菜软腐病病原菌鉴定及48份不结球白菜种质资源的抗性评价 |
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| 引用本文：王欢,车舒,韩建军,范加勤,佘旭东,刘照坤.不结球白菜软腐病病原菌鉴定及48份不结球白菜种质资源的抗性评价.植物保护学报,2025,52(2):327-339 |
| DOI：10.13802/j.cnki.zwbhxb.2025.2024097 |
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| 中文摘要:为明确不结球白菜Brassica campestris ssp.chinensis软腐病的病原菌，并建立高效快捷的苗期抗性评估体系，从苏州市田间采集具有典型软腐症状的不结球白菜茎秆样本，采用组织分离法获得纯培养菌株，运用多相分类学方法对分离菌株进行鉴定；优化苗期抗性评价体系中接种方法、接种菌株、接种浓度及接种后调查时间等因素，并利用优化的苗期抗性评价体系对48份不结球白菜种质资源进行苗期抗性评价。结果显示：从不结球白菜感病茎秆组织中分离纯化获得1株菌株，命名为SZCX；经多相分类学方法鉴定，引起不结球白菜软腐病的病原菌为巴西果胶杆菌Pectobacte‐rium brasiliense； SZCX菌株能分泌多种胞外植物细胞壁降解酶，寄主广泛，可侵染马铃薯Solanumtuberosum、胡萝卜Daucus carota、萝卜Raphanus sativus、莴苣Lactuca sativa和芹菜Apium graveo‐lens等多种蔬菜；优化的苗期抗性评价体系中接种菌株为巴西果胶杆菌，接种浓度为1×108 CFU/mL，接种方法为伤口法接种，接种后调查时间为幼苗接种24 h后；经苗期抗性评价体系评价，在48份不结球白菜种质资源中，3份对软腐病表现为抗病，4份表现为中抗，其余均为感病或高感材料。 |
| 中文关键词:不结球白菜 软腐病 病原菌 鉴定 巴西果胶杆菌 抗性水平 评价 |
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| Identification of a pathogenic bacterium causing bacterial soft rot in nonheading Chinese cabbages and resistance evaluation of 48 non-heading Chinese cabbage germplasm resources |
| Author Name | Affiliation | E-mail | | Wang Huan | Suzhou Academy of Agricultural Sciences, Suzhou 215155, Jiangsu Province, China | | | Che Shu | College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China
Institute of Plant Protection, Jiangxi Agricultural Academy of Sciences, Nanchang 330200, Jiangxi Province, China | | | Han Jianjun | Suzhou Academy of Agricultural Sciences, Suzhou 215155, Jiangsu Province, China | | | Fan Jiaqin | College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China | | | She Xudong | Suzhou Academy of Agricultural Sciences, Suzhou 215155, Jiangsu Province, China | | | Liu Zhaokun | Suzhou Academy of Agricultural Sciences, Suzhou 215155, Jiangsu Province, China | saaslzk@qq.com |
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| Abstract:To identify the pathogen causing soft rot and develop an efficient seedling resistance evaluation system, stem samples showing typical symptoms were collected from non-heading Chinese cabbage fields in suzhou City, and pure culture strains were isolated from the samples using a tissue isolation method and identified through polyphasic taxonomy approaches; the seedling resistance evaluation system was optimized for factors such as inoculation method, strain, concentration, and post-inoculation investigation duration. The optimized system was used to assess seedling resistance in 48 non-heading Chinese cabbage germplasm resources. Results showed that one strain, named SZCX, isolated and purified from diseased stem tissues, was identified as Pectobacterium brasiliense with polyphasic taxonomy, which caused soft rot on non-heading Chinese cabbages. The SZCX strain produced extracellular plant cell wall-degrading enzymes and had a broad host range, infecting various vegetables such as potato, carrot, radish, lettuce, and celery. In the seedling resistance evaluation system, P. brasiliense was inoculated at a concentration of 1×108 CFU/mL using a wound inoculation method, with a 24-hour postinoculation investigation period. Among 48 non-heading Chinese cabbage germplasm resources, three of them showed resistance to soft rot, and four of them displayed moderate resistance, while the rest were categorized as susceptible or highly susceptible. |
| keywords:non-heading Chinese cabbage soft rot pathogen identification Pectobacterium brasiliense resistance level evaluation |
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