欢迎访问《植物保护学报》网站！

首页 | 期刊简介 |
编委会
编委会
青年编委会
| 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English

抑肽酶对防治梨小食心虫的两种植物源农药的增效作用

引用本文：李俊杰,杨晓燕,唐慧琳,高俊恒,郭子坤,郭春阳,王冬寒,叶佳成,袁向群,李怡萍.抑肽酶对防治梨小食心虫的两种植物源农药的增效作用.植物保护学报,2025,52(1):105-112

DOI：10.13802/j.cnki.zwbhxb.2025.2024078

摘要点击次数:

全文下载次数:

作者	单位	E-mail
李俊杰	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
杨晓燕	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
唐慧琳	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
高俊恒	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
郭子坤	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
郭春阳	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
王冬寒	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
叶佳成	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
袁向群	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
李怡萍	西北农林科技大学植物保护学院, 农业农村部西北黄土高原作物有害生物综合治理重点实验室, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100	liyiping@nwsuaf.edu.cn

中文摘要:为提高植物源药剂对梨小食心虫Grapholita molesta的防治效果，测定饲喂含抑肽酶的人工饲料后梨小食心虫2龄幼虫中肠内总蛋白酶、胰蛋白酶和胰凝乳蛋白酶的活性，采用饲料混毒法测定0.20 mg/L抑肽酶对1%印楝素水分散粒剂和0.3%苦参碱水剂的增效作用。结果表明：饲喂含0.20 mg/L抑肽酶的人工饲料12 h后，梨小食心虫2龄幼虫中肠内总蛋白酶、胰蛋白酶和胰凝乳蛋白酶的活性均与对照无显著差异；饲喂36 h后，这3种蛋白酶的活性均较对照显著下降。当0.20 mg/L抑肽酶分别与LC₁₀、LC₃₀、LC₅₀的1%印楝素水分散粒剂混配饲喂后，梨小食心虫2龄幼虫的实际死亡率分别为29.16%、43.06%和66.67%，均显著高于预测死亡率；当0.20 mg/L抑肽酶分别与L_C10、LC₃₀和LC₅₀的0.3%苦参碱水剂混配饲喂后，梨小食心虫2龄幼虫的实际死亡率分别为44.44%、58.33%和68.89%，均显著高于预测死亡率。表明抑肽酶通过抑制梨小食心虫中肠蛋白酶活性对印楝素或苦参碱产生增效作用。

中文关键词:梨小食心虫抑肽酶苦参碱印楝素增效作用

Synergistic effect of aprotinin with two botanical pesticides against oriental fruit moth Grapholita molesta

Author Name	Affiliation	E-mail
Li Junjie	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Yang Xiaoyan	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Tang Huilin	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Gao Junheng	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Guo Zikun	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Guo Chunyang	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Wang Donghan	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Ye Jiacheng	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Yuan Xiangqun	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Li Yiping	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China	liyiping@nwsuaf.edu.cn

Abstract:To improve the control effect of botanical pesticides on oriental fruit moth Grapholita molesta, the activities of total protease, trypsin and chymotrypsin in the midgut of the second larvae of G. molesta were measured after feeding an artificial diet containing 0.20 mg/L aprotinin. The synergistic effect of 0.20 mg/L aprotinin on 1% azadirachtin WG and 0.3% AS was determined by feeding on the diet containing these toxic substances. The results showed that after feeding the artificial diet containing aprotinin for 12 h, the activities of total protease, trypsin and chymotrypsin in the midgut of the second instar larvae were not significantly different from the control group. After feeding for 36 h, the activities of these three proteases decreased significantly compared with the control group. When 0.20 mg/L aprotinin was mixed with LC₁₀, LC₃₀ and LC₅₀ of 1% azadirachtin WG, the actual mortality rates were 29.16%, 43.06% and 66.67%, respectively, which were significantly higher than the predicted mortality. When 0.20 mg/L aprotinin was mixed with LC₁₀, LC₃₀ and LC₅₀ of 0.3% matrine AS, the actual mortality rates were 44.44%, 58.33% and 68.89%, respectively, which were significantly higher than the predicted mortality. These results indicate that aprotinin has a synergistic effect on azadirachtin or matrine by inhibiting the activity of the midgut proteases in G. molesta.

keywords:Grapholita molesta aprotinin matrine azadirachtin synergist effect

查看全文查看/发表评论下载PDF阅读器