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α-茄碱对马铃薯块茎蛾生长发育及代谢解毒的影响

引用本文：闫寒,董文博,高玉林,侯清芳,周钰佳,陈昱儒,刘明月,李芬,吴少英.α-茄碱对马铃薯块茎蛾生长发育及代谢解毒的影响.植物保护学报,2024,51(6):1438-1448

DOI：10.13802/j.cnki.zwbhxb.2024.2024052

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作者	单位	E-mail
闫寒	海南大学三亚南繁研究院, 三亚 572025
董文博	海南大学三亚南繁研究院, 三亚 572025 海南大学热带农林学院, 儋州 571737
高玉林	中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193
侯清芳	海南大学三亚南繁研究院, 三亚 572025 海南大学热带农林学院, 儋州 571737
周钰佳	海南大学三亚南繁研究院, 三亚 572025
陈昱儒	海南大学三亚南繁研究院, 三亚 572025
刘明月	海南大学三亚南繁研究院, 三亚 572025
李芬	海南大学三亚南繁研究院, 三亚 572025 海南大学热带农林学院, 儋州 571737	lifen2010happy@sina.com
吴少英	海南大学三亚南繁研究院, 三亚 572025 海南大学热带农林学院, 儋州 571737	wsywsy6000@hainanu.edu.cn

中文摘要:为有效防治世界性检疫害虫马铃薯块茎蛾Phthorimaea operculella，测定不同浓度α-茄碱胁迫下马铃薯块茎蛾生长发育、解毒酶活性和细胞色素P450（cytochrome P450，CYP450）基因表达量的变化，选择相对表达量差异较显著的2个CYP450基因进行RNA干扰试验，测定干扰这2个CYP450基因后，马铃薯块茎蛾存活率、体重和龄期的变化。结果显示：α-茄碱可以抑制马铃薯块茎蛾幼虫的生长发育；α-茄碱能够诱导马铃薯块茎蛾幼虫体内CYP450酶和谷胱甘肽S-转移酶活性上升，同时抑制乙酰胆碱酯酶与羧酸酯酶活性。在α-茄碱胁迫下，马铃薯块茎蛾体内6个CYP450基因在肠道中高表达，且随着α-茄碱浓度升高，其表达量总体呈现先上升后下降的趋势。干扰CYP340CQ1和CYP6AE221基因后用α-茄碱饲喂马铃薯块茎蛾，其存活率、幼虫体重和3~4龄幼虫期均较对照发生变化。表明CYP450基因在马铃薯块茎蛾对α-茄碱的代谢解毒中可能发挥着重要作用。

中文关键词:马铃薯块茎蛾 α-茄碱生长发育解毒酶 RNA干扰

Effects of α-solanine on growth, development and metabolic detoxification of potato tuber moth Phthorimaea operculella

Author Name	Affiliation	E-mail
Yan Han	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China
Dong Wenbo	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, Hainan Province, China
Gao Yulin	State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Hou Qingfang	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, Hainan Province, China
Zhou Yujia	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China
Chen Yuru	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China
Liu Mingyue	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China
Li Fen	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, Hainan Province, China	lifen2010happy@sina.com
Wu Shaoying	College of Breeding and Multiplication, Hainan University, Sanya 572025, Hainan Province, China School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, Hainan Province, China	wsywsy6000@hainanu.edu.cn

Abstract:To effectively manage and control worldwide quarantine pest potato tuber moth Phthorimaea operculella, the effects of α-solanine at different concentrations on the growth, development, detoxification enzyme activity, and cytochrome P450 (CYP450) gene expression of P. operculella were investigated. Two CYP450 genes with significant relative expression changes were selected for RNA interference (RNAi) experiments. Changes in survival rate, body weight, and developmental stage of P. operculella were analyzed after interference with these two CYP450 genes. The results showed that α-solanine inhibited the growth and development of P. operculella larvae. α-solanine induced an increase in the activity of CYP450 enzymes and glutathione S-transferase, while it inhibited the activity of acetyl-cholinesterase and carboxylesterase. Under α-solanine stress, six CYP450 genes were highly expressed in larval intestines, with expression levels generally increasing initially and then declining as α-solanine concentration increased. RNAi targeting of CYP340CQ1 and CYP6AE221, followed by α-solanine feeding, resulted in significant changes in survival rate, larval weight, and 3rd to 4th instar larval stage of P. operculella. These findings indicate that CYP450 genes play an essential role in the detoxification and metabolism of α-solanine in P. operculella. This study provides insights into the detoxification mechanisms of α-solanine and offers new strategies for the prevention and control of P. operculella.

keywords:Phthorimaea operculella α-solanine growth and development detoxification enzyme RNA interference

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