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锈色粒肩天牛化学感受基因鉴定与分析

引用本文：周敏,梁志,郑永鑫,顾天滋,张胜男,张龙娃.锈色粒肩天牛化学感受基因鉴定与分析.植物保护学报,2024,51(6):1413-1426

DOI：10.13802/j.cnki.zwbhxb.2024.2024019

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作者	单位	E-mail
周敏	安徽农业大学林学与园林学院, 安徽省林木资源培育重点实验室, 真菌生物技术教育部工程研究中心, 安徽省微生物防治重点实验室, 合肥 230036
梁志	安徽农业大学林学与园林学院, 安徽省林木资源培育重点实验室, 真菌生物技术教育部工程研究中心, 安徽省微生物防治重点实验室, 合肥 230036
郑永鑫	安徽农业大学林学与园林学院, 安徽省林木资源培育重点实验室, 真菌生物技术教育部工程研究中心, 安徽省微生物防治重点实验室, 合肥 230036
顾天滋	安徽农业大学林学与园林学院, 安徽省林木资源培育重点实验室, 真菌生物技术教育部工程研究中心, 安徽省微生物防治重点实验室, 合肥 230036
张胜男	安徽农业大学林学与园林学院, 安徽省林木资源培育重点实验室, 真菌生物技术教育部工程研究中心, 安徽省微生物防治重点实验室, 合肥 230036
张龙娃	安徽农业大学林学与园林学院, 安徽省林木资源培育重点实验室, 真菌生物技术教育部工程研究中心, 安徽省微生物防治重点实验室, 合肥 230036	zhanglw@ahau.edu.cn

中文摘要:为鉴定锈色粒肩天牛Apriona swainsoni的化学感受基因，利用Illumina Novaseq 6000平台对其雌雄成虫触角、口器、腹部生殖节、前足跗节进行转录组测序，从中筛选获得化学感受基因并进行生物信息学和系统进化分析，同时测定其组织表达模式，进一步通过序列比对和构建进化树分析锈色粒肩天牛、同属共寄主的桑天牛Apriona germari以及其他鞘翅目昆虫化学感受基因的同源性和系统发育关系。结果表明：从锈色粒肩天牛各组织中共获得32 242条unigene，从中鉴定得到14个味觉受体（gustatory receptor，GR）基因、9个离子型受体（ionotropic receptor，IR）基因和39个气味受体（odorant receptor，OR）基因；其中，AswaGR5、AswaGR7、AswaGR12、AswaGR13聚集在果糖受体家族，AswaGR4聚集在糖受体家族，AswaIR9和AswaIR8为IR共受体基因，且这3类基因均与桑天牛的化学感受基因高度同源。大部分化学感受基因在锈色粒肩天牛触角或口器中高表达，不同基因呈现出不同的组织表达特异性，如AswaGR5、AswaIR2、AswaIR7、AswaOR3、AswaOR5、AswaOR6、AswaOR8、AswaOR18、AswaOR25、AswaOR28、AswaOR36和AswaOR38基因仅于雌雄成虫触角中有表达，AswaGR1基因在雄成虫口器中显著高表达。锈色粒肩天牛与桑天牛的AswaGR1/AgerGR6、AswaIR9/AgerIR9、AswaOR13/AgerOR22和AswaOrco/AgerOR25等化学感受基因有极高的同源性，推测这些基因可能在寄主定位以及生态位分隔中发挥着重要作用。

中文关键词:锈色粒肩天牛化学感受基因嗅觉受体表达模式同源性分析

Identification and analysis of chemosensory genes in rusty-spotted longhorn beetle Apriona swainsoni (Coleoptera: Cerambycidae)

Author Name	Affiliation	E-mail
Zhou Min	Anhui Provincial Key Laboratory of Microbial Control Engineering Research Center of Fungal Biotechnology, Ministry of Education Anhui Provincial Key Laboratory of Forest Resources and Silviculture School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, Anhui Province, China
Liang Zhi	Anhui Provincial Key Laboratory of Microbial Control Engineering Research Center of Fungal Biotechnology, Ministry of Education Anhui Provincial Key Laboratory of Forest Resources and Silviculture School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, Anhui Province, China
Zheng Yongxin	Anhui Provincial Key Laboratory of Microbial Control Engineering Research Center of Fungal Biotechnology, Ministry of Education Anhui Provincial Key Laboratory of Forest Resources and Silviculture School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, Anhui Province, China
Gu Tianzi	Anhui Provincial Key Laboratory of Microbial Control Engineering Research Center of Fungal Biotechnology, Ministry of Education Anhui Provincial Key Laboratory of Forest Resources and Silviculture School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, Anhui Province, China
Zhang Shengnan	Anhui Provincial Key Laboratory of Microbial Control Engineering Research Center of Fungal Biotechnology, Ministry of Education Anhui Provincial Key Laboratory of Forest Resources and Silviculture School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, Anhui Province, China
Zhang Longwa	Anhui Provincial Key Laboratory of Microbial Control Engineering Research Center of Fungal Biotechnology, Ministry of Education Anhui Provincial Key Laboratory of Forest Resources and Silviculture School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, Anhui Province, China	zhanglw@ahau.edu.cn

Abstract:To identify chemosensory genes inrusty-spotted longhorn beetle Apriona swainsoni, tran-scriptome sequencing was performed on antennae, mouthparts, reproductive segments, and prothoracic tarsi of both male and female adults using the Illumina Novaseq 6000 platform. Chemosensory genes were screened and analyzed through bioinformatics and phylogenetic tree analysis. Tissue-specific expression patterns were determined based on fragments per kilobase per million mapped fragments (FP-KM) values. Sequence alignment and phylogenetic analyses were also conducted to examine the homology and phylogenetic relationships of chemoreceptor genes among A. swainsoni, its congeneric host Apriona germari, and other Coleoptera species. The results showed that 32 242 unigenes, among which 14 gustatory receptors (GRs), nine ionotropic receptors (IRs), and 39 odorant receptors (ORs) were iden‐tified. Phylogenetic analysis indicated that AswaGR5, AswaGR7, AswaGR12 and AswaGR13 clustered in the fructose receptor subfamily, AswaGR4 clustered in the glucose receptor subfamily. AswaIR9 and AswaIR8 were identified as IR co-receptor genes. These three types of chemosensory genes showed high homology with those of A. germari. Most chemosensory genes were highly expressed in the anten-nae or mouthparts, with tissue-specific expression patterns observed. For instance, AswaGR5, AswaIR2, AswaIR7, AswaOR3, AswaOR5, AswaOR6, AswaOR8, AswaOR18, AswaOR25, AswaOR28, AswaOR36 and AswaOR38 were exclusively expressed in the antennae of both sexes, whereas AswaGR1 showed significantly higher expression in the male mouthparts. Notably, chemosensory genes such as AswaGR1/ AgerGR6, AswaIR9/AgerIR9, AswaOR13/AgerOR22, and AswaOrco/AgerOR25 exhibited high homology between A. swainsoni and A. germari, suggesting their potential roles in host location and ecological niche segregation.

keywords:Apriona swainsoni chemosense gene olfactory receptor expression pattern homology analysis

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