| 不同遗传变异下稻曲病菌的生物学特性及对杀菌剂的敏感性分析 |
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| 引用本文：方安菲,杨雯钰,张世芸,王林栋,张瑞璇,杨军.不同遗传变异下稻曲病菌的生物学特性及对杀菌剂的敏感性分析.植物保护学报,2024,51(6):1306-1316 |
| DOI：10.13802/j.cnki.zwbhxb.2024.2024018 |
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| 中文摘要:为明确稻曲病菌Ustilaginoidea virens在不同遗传变异下的生物学特性及对不同杀菌剂的敏感性，对收集的27株不同单倍型稻曲病菌菌株进行遗传进化关系分析和生长速率及产孢能力测定，并分析其对不同类型杀菌剂的敏感性及相关性。结果显示：27株稻曲病菌菌株被划分为2个遗传亚群I和II，其生长速率介于1.905~2.845 mm/d之间，产孢量介于6.667×104~563.333×104个/mL之间。甲氧基丙烯酸酯（quinone outside inhibitor，QoI）类杀菌剂吡唑醚菌酯和嘧菌酯对稻曲病菌的EC50分别介于0.023~0.804 μg/mL和0.032~0.254 μg/mL之间，甾醇脱甲基抑制剂（demethylation inhibitor，DMI）类杀菌剂咪鲜胺和戊唑醇对稻曲病菌的EC50分别介于0.022~1.145 μg/mL和0.009~0.016 μg/mL之间，琥珀酸脱氢酶抑制剂（succinate dehydrogenase inhibitor，SDHI）类杀菌剂啶酰菌胺和噻呋酰胺对稻曲病菌的EC50分别介于29.239~85.779 μg/mL和118.804~286.860 μg/mL之间，表明不同单倍型菌株之间的生物学特性差异明显，且对QoI和DMI类杀菌剂的敏感性较对SDHI类杀菌剂更高。亚群I和II对啶酰菌胺和噻呋酰胺的敏感性存在显著差异，表明不同的遗传进化方向可能影响稻曲病菌对SDHI类杀菌剂的敏感性。供试菌株的生长速率与其对戊唑醇的敏感性呈显著负相关；产孢能力与其对咪鲜胺的敏感性呈显著负相关；致病性与其对吡唑醚菌酯以及咪鲜胺的敏感性呈显著正相关，而与其对嘧菌酯的敏感性呈显著负相关。建议选择QoI和DMI类杀菌剂对田间出现的强致病性稻曲病菌群体进行防治，可提高对稻曲病的防治效果。 |
| 中文关键词:稻曲病菌 生长速率 产孢 杀菌剂敏感性 相关性 |
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| Biological characteristics of the rice false smut pathogen Ustilaginoidea virens with different genetic variations and their sensitivities to fungicides |
| Author Name | Affiliation | E-mail | | Fang Anfei | College of Plant Protection, Southwest University, Chongqing 400715, China | | | Yang Wenyu | College of Plant Protection, Southwest University, Chongqing 400715, China | | | Zhang Shiyun | College of Plant Protection, Southwest University, Chongqing 400715, China | | | Wang Lindong | College of Plant Protection, Southwest University, Chongqing 400715, China | | | Zhang Ruixuan | College of Plant Protection, Southwest University, Chongqing 400715, China | | | Yang Jun | Institute of Wetland Agriculture and Ecology, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China | yangjuncol@163.com |
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| Abstract:To clarify the biological characteristics of 27 different haplotype Ustilaginoidea virens isolates with different genetic variations and their sensitivities to fungicides, their genetic evolution relationship was constructed, and their growth rates, sporulation and sensitivities to different types of fungicides were determined, and their correlation tests were performed. The phylogeny tree showed that 27 haplotype U. virens isolates were divided into two genetic subgroups, I and II. The growth rates of the 27 haplotype isolates ranged from 1.905 to 2.845 mm/d, and the sporulation ranged from 6.667×104 to 563.333×104 spores per milliliter. The EC50 values of 27 isolates tested to quinone outside inhibitors (QoIs), pyraclostrobin and azoxystrobin, ranged from 0.023 to 0.804 μg/mL and 0.032 to 0.254 μg/mL, respectively, and the EC50 values for demethylation inhibitors (DMIs), prochloraz and tebuconazole, ranged from 0.022 to 1.145 μg/mL and 0.009 to 0.016 μg/mL, respectively. The EC50 values for succinate dehydrogenase inhibitors (SDHIs), boscalid and thifluzamide, ranged from 29.239 to 85.779 μg/mL and 118.804 to 286.860 μg/mL, respectively. These results suggested that the biological characteristics of different haplotype isolates were different, and they were more sensitive to QoIs and DMIs than SDHIs. Furthermore, significant sensitivity differences for boscalid and thifluzamide were detected between subgroups I and II, indicating that different genetic evolution directions may affect the sensitivity of U. virens to SDHIs. The growth rate of the tested isolates was negatively correlated with the sensitivity of isolates to tebuconazole. Sporulation was negatively correlated with the sensitivity of U. virens to prochloraz. The pathogenicity was positively correlated with the sensitivities of U. virens to pyraclos-trobin and prochloraz but negatively correlated with the sensitivity to azoxystrobin. Taken together, the QoIs and DMIs are recommended to be used for improving the efficacy of control of the U. virens populations with strong virulence in the field. |
| keywords:Ustilaginoidea virens growth rate sporulation sensitivity to fungicide correlation |
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