• 首页 | 期刊简介  | 
    编委会
    编委会
    青年编委会
     | 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English
广东省不同稻区稻瘟病菌生理小种鉴定及无毒基因分析
点此下载全文
引用本文:汪文娟,陈凯玲,杨健源,封金奇,朱小源,苏菁.广东省不同稻区稻瘟病菌生理小种鉴定及无毒基因分析.植物保护学报,2024,51(3):645-653
DOI:10.13802/j.cnki.zwbhxb.2024.2023090
摘要点击次数:
全文下载次数:
作者单位E-mail
汪文娟 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640  
陈凯玲 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640  
杨健源 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640  
封金奇 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640  
朱小源 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640  
苏菁 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640 1835795725@qq.com 
中文摘要:为明确广东省不同稻区稻瘟病菌Magnaporthe oryzae生理小种与无毒基因的类型与分布,利用7个中国鉴别品种、11个抗稻瘟病单基因系及3个广东省优质抗源稻种三黄占2号、青六矮和珍桂矮,采用苗期喷雾接种方法对2018—2019年分别从广东省不同稻区感染稻瘟病的不同栽培稻品种上分离获得的368株稻瘟病菌单孢菌株进行生理小种鉴定与无毒基因分析。结果显示,利用中国鉴别品种共鉴定出 6 群 30 个生理小种,其中优势种群为 ZB 和 ZC,出现频率分别为 44.29% 和43.48%,优势生理小种为ZB13和ZC13,出现频率分别为26.90%和29.35%。利用11个水稻抗稻瘟病单基因系鉴定出测试菌株对含有Pi9、Pi1、Pik-hPi50这4个水稻抗稻瘟病单基因系的无毒性频率均大于80.00%;来自粤北、粤西和珠三角3个稻区的稻瘟病菌对抗源稻种三黄占2号的毒性频率低于11.76%,对珍桂矮的毒性频率大于69.41%。表明2018—2019年广东省稻瘟病菌群体生理小种结构复杂,多样性丰富,在粤北、粤西和珠三角稻区可利用的抗性基因一致,可推广使用含抗性基因Pi9、Pi50、Pik-hPi1的水稻品种;三黄占2号抗性好,抗谱较宽,可作为广东省抗稻瘟病种质资源加以利用。
中文关键词:水稻  稻瘟病菌  生理小种  优势小种  无毒基因型
 
Identification of physiological races and analysis of avirulence genes of rice blast fungus Magnaporthe oryzae in different rice regions of Guangdong Province
Author NameAffiliationE-mail
Wang Wenjuan Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China  
Chen Kailing Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China  
Yang Jianyuan Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China  
Feng Jinqi Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China  
Zhu Xiaoyuan Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China  
Su Jing Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China 1835795725@qq.com 
Abstract:In order to understand the types and distribution of physiological races and avirulence genes of rice blast fungus Magnaporthe oryzae in different rice-growing regions of Guangdong Province, the method of spray inoculation was used at the seedling stage of identified varieties. A total of 368 singlespore strains isolated from different cultivated rice varieties infected with rice blast disease in different rice regions of Guangdong Province from 2018 to 2019 were tested for their pathogenicity and the corresponding avirulence genotypes, against seven Chinese differential cultivars and 11 blast monogenic lines, and three Guangdong high-quality resistant rice varieties Sanhuangzhan 2, Qingliu’ai, and Zhengui’ai. The results showed that six groups and 30 Chinese physiological races were identified and the predominant populations were ZB and ZC, with frequency of 44.29% and 43.48%, respectively. The predominant physiological races were ZB13 and ZC13, with frequencies of 26.90% and 29.35%, respectively. Using 11 blast monogenic lines for pathogenicity analysis, the results showed that the average avirulent frequency of rice blast monogenic lines containing Pi9, Pi1, Pik-h, and Pi50 from the test strains was greater than 80.00%. Further pathogenicity analysis was conducted on populations of rice blast fungus isolated from different cultivated rice varieties. The pathogenicity test results showed that the virulence frequency of rice blast fungus from three rice regions to the resistant rice variety Sanhuangzhan 2 was lower than 11.76%, and the virulence frequency to Zhengui’ai was greater than 69.41%. This indicated that the physiological race structure of rice blast fungus population in Guangdong Province from 2018 to 2019 was complicated with rich diversity. The resistance genes available in the rice-growing areas of northern and western Guangdong, and the Pearl River Delta were consistent, and rice varieties containing resistance genes Pi9, Pi50, Pik-h, and Pi1 could be promoted for use in the near future; Sanhuangzhan 2 possessed high resistance and a wide resistance spectrum, thus could be used as a blast-resistant germplasm resource in Guangdong Province.
keywords:rice  Magnaporthe oryzae  physiological race  predominant race  genotype of avirulence
查看全文  查看/发表评论  下载PDF阅读器
您是本站第  9036414 版权所有:植物保护学报    京ICP备05006550号-2  
主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部
电话:010-62732528 电子邮件:zbxb@cau.edu.cn
技术支持:北京勤云科技发展有限公司