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137份云南省地方稻抗白叶枯病多菌系多基因鉴定

引用本文：李玉琢,陈玲,张敦宇,管娟,张云,钟巧芳,殷富有,李馨,杨顺发,王波,程在全,罗琼,肖素勤.137份云南省地方稻抗白叶枯病多菌系多基因鉴定.植物保护学报,2023,50(4):894-904

DOI：10.13802/j.cnki.zwbhxb.2023.2022035

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作者	单位	E-mail
李玉琢	云南农业大学植物保护学院, 昆明 650500 云南省农业科学院生物技术与种质资源研究所, 昆明 650205
陈玲	云南省农业科学院生物技术与种质资源研究所, 昆明 650205
张敦宇	云南省农业科学院生物技术与种质资源研究所, 昆明 650205
管娟	云南大学资源植物研究院, 昆明 650504
张云	云南省农业科学院生物技术与种质资源研究所, 昆明 650205
钟巧芳	云南省农业科学院生物技术与种质资源研究所, 昆明 650205
殷富有	云南省农业科学院生物技术与种质资源研究所, 昆明 650205
李馨	元江乡村振兴发展中心, 玉溪 653300
杨顺发	元江乡村振兴发展中心, 玉溪 653300
王波	云南省农业科学院生物技术与种质资源研究所, 昆明 650205
程在全	云南省农业科学院生物技术与种质资源研究所, 昆明 650205
罗琼	云南农业大学植物保护学院, 昆明 650500	qiongbf@aliyun.com
肖素勤	云南省农业科学院生物技术与种质资源研究所, 昆明 650205	xiaosuqin227@126.com

中文摘要:为选育水稻抗性品种和发掘抗白叶枯病基因，以来自中国、日本和菲律宾的9株革兰氏阴性菌黄单胞菌水稻变种Xanthomonas oryzae pv.oryzae菌株为供试菌株评价137份云南省地方稻种质资源对白叶枯病菌的抗性，同时检测供试材料中含14个已克隆抗白叶枯病基因的情况。结果显示，供试材料中至少对1株黄单胞菌水稻变种菌株有抗性的材料有104份，抗1、2、3、4、5、6、7、8和9株黄单胞菌水稻变种菌株的材料分别有25、19、14、19、11、6、5、2和3份；其中白杂一号、珍白18、麻旱谷、老来红、晋糯、香谷、毫冷薅、齐头蚂蚱谷、九谷和来之不易10份材料抗谱较广。大部分抗性材料携带1~7个白叶枯病抗性基因或其同源基因，未检测到含有Xa7、xa13、Xa21和Xa45（t）基因或其同源基因的材料。供试材料携带的抗性基因数量与抗性水平均呈极显著相关。

中文关键词:云南地方稻白叶枯病表型鉴定基因分析广谱抗病

Multi-bacterial lines and multi-gene identification of the bacterial blight pathogen in 137 rice landraces from Yunnan Province, China

Author Name	Affiliation	E-mail
Li Yuzhuo	School of Plant Protection, Yunnan Agricultural University, Kunming 650500, Yunnan Province, China Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Chen Ling	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Zhang Dunyu	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Guan Juan	School of Resource Plants, Yunnan University, Kunming 650504, Yunnan Province, China
Zhang Yun	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Zhong Qiaofang	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Yin Fuyou	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Li Xin	Yuanjiang Rural Revitalization and Development Centre, Yuxi 653300, Yunnan Province, China
Yang Shunfa	Yuanjiang Rural Revitalization and Development Centre, Yuxi 653300, Yunnan Province, China
Wang Bo	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Cheng Zaiquan	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Luo Qiong	School of Plant Protection, Yunnan Agricultural University, Kunming 650500, Yunnan Province, China	qiongbf@aliyun.com
Xiao Suqin	Biotechnology & Genetic Resources Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China	xiaosuqin227@126.com

Abstract:To breed resistance rice varieties and mining for the bacterial blight resistance genes,nine represent Xanthomonas oryzae pv. oryzae (Xoo) strains from China, Japan and Philippines were inoculated with 137 rice landraces from Yunnan Province to evaluate the phenotypic resistance to bacterial blight, and 14 cloned bacterial blight resistance genes contained in the tested materials were tested. The results showed that 104 materials were resistant to at least one Xoo strain, and the numbers of materials resistant to one through nine Xoo strains were 25, 19, 14, 19, 11, 6, 5, 2 and 3 accessions, respectively. Among them, materials Baizayihao, Zhenbai 18, Mahangu, Laolaihong, Jinnuo, Xianggu, Haolenghao, Qitoumazhagu, Jiugu, and Laizhibuyi 10 had a broad resistance spectrum. Most of the resistance materials carried one to seven bacterial blight resistance genes or their homologs examined, and four resistance allelic fragments of Xa7, xa13, Xa21 and Xa45(t) were not detected in the resistant materials. The number of resistance genes carried by the tested materials was all significantly associated with the resistance level.

keywords:Yunnan landrace bacterial blight phenotype identification genotypic analysis broadspectrum resistance

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