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天津市烟粉虱隐种鉴定及其携带TYLCV、内共生菌情况和抗药性监测

引用本文：胡明鑫,杨泽众,李妍,张楠,王芳,白义川,焦晓国,张李香,刘佰明.天津市烟粉虱隐种鉴定及其携带TYLCV、内共生菌情况和抗药性监测.植物保护学报,2023,50(1):101-110

DOI：10.13802/j.cnki.zwbhxb.2023.2021029

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作者	单位	E-mail
胡明鑫	黑龙江大学现代农业与生态环境学院, 哈尔滨 150080 天津市农业科学院植物保护研究所, 天津 300384
杨泽众	天津市农业科学院植物保护研究所, 天津 300384
李妍	天津市农业科学院植物保护研究所, 天津 300384 湖北大学生命科学学院, 武汉 430062
张楠	天津市农业科学院植物保护研究所, 天津 300384
王芳	天津市农业科学院植物保护研究所, 天津 300384
白义川	天津市农业科学院植物保护研究所, 天津 300384
焦晓国	湖北大学生命科学学院, 武汉 430062
张李香	黑龙江大学现代农业与生态环境学院, 哈尔滨 150080	fjszhanglx@126.com
刘佰明	天津市农业科学院植物保护研究所, 天津 300384	baimingliu@126.com

中文摘要:为明确天津市烟粉虱Bemisia tabaci隐种的类别及其寄主适应性、传毒能力、携带内共生菌情况和抗药性,采用mtCOI酶切法对从武清、西青、蓟州和宁河4个区的番茄、黄瓜及辣椒3种寄主上采集的12个烟粉虱种群进行隐种鉴定,采用PCR检测其携带番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)和内共生菌情况,并采用浸叶法测定其对4种常用药剂的抗性。结果表明,采集的烟粉虱种群以MED隐种为主,占所有检测个体的93.33%,有3个种群为MED和MEAM1隐种混合发生。所有检测个体中有36.25%的个体携带TYLCV,在6个种群中检测到TYLCV,其中5个种群有超过50%的个体携带TYLCV。在12个种群中共检测到Hamiltonella、立克次氏体Rickettsia、Cardinium和杀雄菌属Arsenophnus共4种内共生菌,携带个体比例分别为90.63%、48.96%、43.75%和8.33%,进一步对内共生菌协同感染情况进行分析,发现有HARC、HRC、HAC、HR、HC和AC共6个协同感染型,感染率分别为4.17%、28.13%、3.13%、14.58%、5.21%和1.04%。阿维菌素对天津市烟粉虱种群的毒力普遍较高,LC₅₀介于0.05~0.66mg/L之间,噻虫嗪的毒力则普遍较低,LC₅₀介于267.63~2998.80mg/L之间。4种药剂对其中9个种群的毒力顺序为阿维菌素>溴氰虫酰胺>吡虫啉>噻虫嗪,对另外3个种群的毒力顺序为阿维菌素>吡虫啉>溴氰虫酰胺>噻虫嗪。

中文关键词:烟粉虱隐种番茄黄化曲叶病毒(TYLCV) 内共生菌抗药性

Cryptic Bemisia tabaci species harboring tomato yellow leaf curl virus and endosymbionts and insecticide resistance monitoring in Tianjin

Author Name	Affiliation	E-mail
HU Ming-xin	College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang Province, China Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
YANG Ze-zhong	Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
LI Yan	Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China School of Life Sciences, Hubei University, Wuhan 430062, Hubei Province, China
ZHANG Nan	Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
WANG Fang	Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
BAI Yi-chuan	Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
JIAO Xiao-guo	School of Life Sciences, Hubei University, Wuhan 430062, Hubei Province, China
ZHANG Li-xiang	College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang Province, China	fjszhanglx@126.com
LIU Bai-ming	Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China	baimingliu@126.com

Abstract:To define the cryptic species of tobacco whitefly Bemisia tabaci, as well as their host fitness,virus transmission, facultative symbiont vectoring and insecticide resistance in Tianjin, the mitochondrial cytochrome oxidase I(mtCOI) gene sequences was used to identify 12 populations of B. tabaci collected from three host plants(tomato, cucumber and pepper) in four districts(Wuqing, Xiqing, Jizhou and Ninghe) in Tianjin. Tomato yellow leaf curl virus(TYLCV) and endosymbionts were detected in these populations by PCR. The resistance to four insecticides was also monitored in the 12 B. tabaci populations. The results indicated that the Mediterranean(MED) species accounted for 93.33% among the total whitefly samples tested, and there were three mixed B. tabaci populations with MED and Middle East-Asia Minor 1(MEAM1). The rate of TYLCV-carrying whiteflies was 36.25% of the total tested samples, and TYLCV was detected in six populations, five of which had a virus-carrying rate of more than 50%. The endosymbionts Hamiltonella, Rickettsia, Cardinium and Arsenophonus of B. tabaci were detected in 12 populations, accounting for 90.63%, 48.96%, 43.75% and 8.33%, respectively. Further analysis of the co-infection of the four endosymbionts indicated that there were six co-infection types, including HARC, HRC, HAC, HR, HC and AC, and the infection rates were 4.17%, 28.13%,3.13%, 14.58%, 5.21% and 1.04%, respectively. Generally, B. tabaci in Tianjin was more sensitive to abamectin(LC₅₀: 0.05-0.66 mg/L), but more resistant to thiamethoxam(LC₅₀: 267.63-2 998.80 mg/L).The toxicity of the four insecticides to nine populations was ranked as followed: avermectin>cyantraniliprole>imidacloprid>thiamethoxam, and to the other three populations, avermectin>imidacloprid>cyantraniliprole>thiamethoxam.

keywords:Bemisia tabaci putative species tomato yellow leaf curl virus(TYLCV) endosymbiont insecticide resistance

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