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复合侵染水茄的两种菜豆金色花叶病毒属病毒基因组结构特征分析

引用本文：赵丽玲,钟静,施章吉,李婷婷,丁铭,张仲凯.复合侵染水茄的两种菜豆金色花叶病毒属病毒基因组结构特征分析.植物保护学报,2020,47(2):355-364

DOI：10.13802/j.cnki.zwbhxb.2020.2019119

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作者	单位	E-mail
赵丽玲	云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明 650223
钟静	云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明 650223
施章吉	云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明 650223
李婷婷	云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明 650223
丁铭	云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明 650223	mingd73@163.com
张仲凯	云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明 650223	zhongkai99@sina.com

中文摘要:为明确水茄Solanum torvum植株叶片邹缩、褪绿是否由菜豆金色花叶病毒属病毒侵染引起，从云南省西双版纳傣族自治州田间采集具有疑似感染症状的水茄植株叶片样品，应用菜豆金色花叶病毒属病毒简并引物和特异性引物进行PCR扩增、克隆和测序，通过生物信息软件分析比较其核苷酸序列特征，并对其进行系统发育分析。结果显示，从采集的疑似病叶中共克隆获得了5条菜豆金色花叶病毒属病毒DNA-A全序列和3条DNA-B全序列，经全序列分析发现，侵染水茄的2种菜豆金色花叶病毒属病毒分离物分别属于中国南瓜曲叶病毒（squash leaf curl China virus，SLCCNV）和野茼蒿黄脉病毒（Crassocephalum yellow vein virus，CraYVV）。SLCCNV水茄分离物的基因组具有典型的菜豆金色花叶病毒属病毒双组分结构特征，与来自泰国的SLCCNV分离物（AB330078）亲缘关系最近，相似性最高达到99.0%；CraYVV水茄分离物的基因组具有典型的菜豆金色花叶病毒属病毒单组分结构特征，与来自云南省景洪市的CraYVV分离物（EF165536）亲缘关系最近，相似性最高达到97.6%。表明水茄是这2种菜豆金色花叶病毒属病毒的新寄主，并首次发现双组分和单组分菜豆金色花叶病毒属病毒可复合侵染水茄。

中文关键词:中国南瓜曲叶病毒野茼蒿黄脉病毒复合侵染水茄基因组结构

Characterization of the genome organization of two begomoviruses mixedly infecting wild eggplant Solanum torvum

Author Name	Affiliation	E-mail
ZHAO Liling	Key Laboratory of Agricultural Biotechnology of Yunnan Province Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan Province, China
ZHONG Jing	Key Laboratory of Agricultural Biotechnology of Yunnan Province Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan Province, China
SHI Zhangji	Key Laboratory of Agricultural Biotechnology of Yunnan Province Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan Province, China
LI Tingting	Key Laboratory of Agricultural Biotechnology of Yunnan Province Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan Province, China
DING Ming	Key Laboratory of Agricultural Biotechnology of Yunnan Province Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan Province, China	mingd73@163.com
ZHANG Zhongkai	Key Laboratory of Agricultural Biotechnology of Yunnan Province Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan Province, China	zhongkai99@sina.com

Abstract:In order to determine whether the crumpling and mosaic symptoms of wild eggplant Solanum torvum were caused by begomoviruses infection, leaf samples of S. torvum plants with suspected symptoms were collected from the fields in Xishuangbanna, Yunnan. PCR amplification of these samples was conducted using degenerate and virus-specific primer pairs of begomoviruses. Five complete DNA-A and three DNA-B genome sequences were obtained by cloning and characterized by genome organization and phylogenetic analyses. Sequence comparison revealed that these isolates identified from S. torvum consisted of squash leaf curl China virus (SLCCNV) and Crassocephalum yellow vein virus (CraYVV). These SLCCNV isolates from S. torvum had a genome organization typical of bipartite begomoviruses and were most closely related to the SLCCNV isolate (AB330078) identified from Thailand (with the highest sequence identity of 99.0%). These CraYVV isolates from S. torvum had a genome organization typical of monopartite begomoviruses and were most closely related to CraYVV-Jinghong (EF165536) (with the highest sequence identity of 97.6%). This study is the first report of S. torvum plants as new hosts of begomoviruses, with bipartite and monopartite begomoviruses simultaneously infecting S. torvum plants.

keywords:squash leaf curl China virus Crassocephalum yellow vein virus mixed infection Solanum torvum genome organization

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