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玫瑰黄链霉菌活性代谢产物RM诱导黄瓜抗病性的信号转导途径
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引用本文:甄丹妹,韩兴,郭景红,孟庆芳,李亚宁,刘大群.玫瑰黄链霉菌活性代谢产物RM诱导黄瓜抗病性的信号转导途径.植物保护学报,2020,47(2):332-338
DOI:10.13802/j.cnki.zwbhxb.2020.2019060
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甄丹妹 河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071001  
韩兴 中国农业科学院研究生院, 北京 100081  
郭景红 河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071001  
孟庆芳 河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071001  
李亚宁 河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071001 yaning22@163.com 
刘大群 河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071001
中国农业科学院研究生院, 北京 100081 
liudaqun@caas.cn 
中文摘要:为明确玫瑰黄链霉菌Streptomyces roseoflavus菌株men-myco-93-63活性代谢产物roflamycoin和men-myco-A(简称RM)诱导黄瓜产生抗病性的信号转导途径,采用高效液相色谱法、分光光度法和实时荧光定量PCR法,测定喷施玫瑰黄链霉菌代谢产物RM后黄瓜叶片中水杨酸含量和脂氧合酶活性,以及水杨酸信号转导途径和乙烯茉莉酸信号转导途径的标志基因——NPR1PR-1aCTR1的表达量。结果表明,喷施玫瑰黄链霉菌活性代谢产物RM后120 h,黄瓜叶片内水杨酸含量达到最高值,为7.22 μg/g,是对照的1.75倍;喷施玫瑰黄链霉菌活性代谢产物RM后12 h,黄瓜叶片内脂氧合酶活性达到最高值,为52.69 U/g,是对照的1.32倍,并且在120 h内整体活性均高于对照;喷施玫瑰黄链霉菌代谢产物RM后,NPR1PR-1a基因的相对表达量上调,其最大值分别为0.99和1.35,分别是对照的2.24倍和12.09倍,但抑制CTR1基因的表达。推测玫瑰黄链霉菌代谢产物RM通过水杨酸通路、乙烯通路和茉莉酸通路共同诱导黄瓜抗病性信号转导途径。
中文关键词:玫瑰黄链霉菌  活性代谢产物  黄瓜  信号转导途径  诱导抗病性
 
Signal transduction pathway of disease resistance induced by active metabolites RM of Streptomyces roseoflavus in cucumber
Author NameAffiliationE-mail
ZHEN Danmei National Engineering Research Center for Agriculture in Northern Mountainous Areas, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei Province, China  
HAN Xing Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China  
GUO Jinghong National Engineering Research Center for Agriculture in Northern Mountainous Areas, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei Province, China  
MENG Qingfang National Engineering Research Center for Agriculture in Northern Mountainous Areas, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei Province, China  
LI Yaning National Engineering Research Center for Agriculture in Northern Mountainous Areas, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei Province, China yaning22@163.com 
LIU Daqun National Engineering Research Center for Agriculture in Northern Mountainous Areas, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei Province, China
Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China 
liudaqun@caas.cn 
Abstract:To study the signal transduction pathway of disease resistance in cucumber induced by active metabolites roflamycoin and men-myco-A (RM) of Streptomyces roseoflavus men-myco-93-63, the content of endogenous salicylic acid (SA), the activity of lipoxygenase (LOX) and the expression of NPR1, PR-1a and CTR1, marker genes of salicylic acid signal transduction pathway and ethylene/jasmonic acid signal transduction pathway in cucumber leaves after sprayed with active metabolites RM of S. roseoflavus were determined by high performance liquid chromatography, spectrophotometry and real-time fluorescence quantitative PCR, respectively. The results showed that the content of SA in cucumber plants increased significantly after sprayed with active metabolites RM of S. roseoflavus, reaching the highest value of 7.22 μg/g at 120 h, 1.75 times that of the control, and the activity of lipoxygenase reaching the highest value of 52.69 U/g at 12 h, 1.32 times that of the control, and the overall activity was higher than the control within 120 h. Sprayed with active metabolites RM of S. roseoflavus could induce up-regulation of NPR1 and PR-1a gene expression, reaching the maximum of 0.99 and 1.35, which were 2.24 and 12.09 times higher than the control at the highest expression levels, respectively, but inhibited CTR1 gene expression. It was presumed that the signal transduction pathway of RM-induced disease resistance was accomplished through salicylic acid pathway, ethylene pathway and jasmonic acid pathway.
keywords:Streptomyces roseoflavus  antimicrobial metabolite  cucumber  signal transduction pathway  induced disease resistance
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