• 首页 | 期刊简介 | 编委会 | 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English
利用表型芯片技术筛选利于枯草芽胞杆菌Bacillus subtilis BAB-1生长与芽胞形成的营养物质
点此下载全文
引用本文:张晓云,郭庆港,鹿秀云,王培培,李社增,马平.利用表型芯片技术筛选利于枯草芽胞杆菌Bacillus subtilis BAB-1生长与芽胞形成的营养物质.植物保护学报,2020,47(2):263-272
DOI:10.13802/j.cnki.zwbhxb.2020.2019099
摘要点击次数:
全文下载次数:
作者单位E-mail
张晓云 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业农村部华北北部作物有害生物综合治理重点实验室, 保定 071000  
郭庆港 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业农村部华北北部作物有害生物综合治理重点实验室, 保定 071000  
鹿秀云 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业农村部华北北部作物有害生物综合治理重点实验室, 保定 071000  
王培培 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业农村部华北北部作物有害生物综合治理重点实验室, 保定 071000  
李社增 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业农村部华北北部作物有害生物综合治理重点实验室, 保定 071000 shezengli@163.com 
马平 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业农村部华北北部作物有害生物综合治理重点实验室, 保定 071000  
中文摘要:为获得枯草芽胞杆菌Bacillus subtilis BAB-1菌株的芽胞高效率生产技术,采用Biolog微生物细胞表型芯片(phenotype microarray,PM)技术分析了适合该菌株细胞生长的营养物质、渗透压和pH等生长环境条件;通过单因素试验分析了5种碳源及5种氮源对该菌株生长及芽胞形成的影响。结果表明,BAB-1菌株能够利用114种碳源、228种氮源、29种磷源及29种硫源作为其营养物质,其中52种碳源、66种氮源、3种硫源及1种磷源能够明显促进该菌株的细胞生长;BAB-1菌株对渗透压的忍受能力较强,在1%~10%氯化钠、3%~6%氯化钾、2%~5%硫酸钠、5%~20%乙二醇、1%~6%甲酸钠、2%~7%尿素、1%~12%乳酸钠、20~200 mmol/L磷酸钠、10~100 mmol/L硝酸钠、10~100 mmol/L硫酸铵中均能较好的生长;BAB-1菌株对pH的耐受范围较广,在4.5 ≤ pH ≤ 10.0条件下均能够生长。葡萄糖与氯化铵分别是最适宜BAB-1菌株生长的碳源与氮源,在不同培养时间其菌体总量均最高,48 h时该菌株的菌体总量分别达到6.90×108 CFU/mL与6.87×108 CFU/mL;培养12 h时,D-木糖、D-核糖、L-阿拉伯糖及熊果苷能够显著提高BAB-1菌株的芽胞形成率,其中L-阿拉伯糖与熊果苷的芽胞形成率较高,分别为61.94%与66.92%;培养12~36 h,L-脯氨酸、L-谷氨酰胺及L-天冬酰胺能够显著提高BAB-1菌株的芽胞形成率,芽胞形成率介于27.88%~82.37%之间。表明表型芯片技术可以应用于枯草芽胞杆菌生长及芽胞形成营养物质的高通量分析及培养工艺的优化中。
中文关键词:枯草芽胞杆菌  表型芯片  芽胞形成  培养工艺
 
Using phenotype microarray to screen the nutrient factors contributing to the growth and sporulation in Bacillus subtilis BAB-1
Author NameAffiliationE-mail
ZHANG Xiaoyun Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China
Integrated Pest Management Center of Hebei Province, Ministry ofAgriculture and RuralAffairs, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China 
 
GUO Qinggang Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China
Integrated Pest Management Center of Hebei Province, Ministry ofAgriculture and RuralAffairs, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China 
 
LU Xiuyun Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China
Integrated Pest Management Center of Hebei Province, Ministry ofAgriculture and RuralAffairs, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China 
 
WANG Peipei Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China
Integrated Pest Management Center of Hebei Province, Ministry ofAgriculture and RuralAffairs, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China 
 
LI Shezeng Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China
Integrated Pest Management Center of Hebei Province, Ministry ofAgriculture and RuralAffairs, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China 
shezengli@163.com 
MA Ping Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China
Integrated Pest Management Center of Hebei Province, Ministry ofAgriculture and RuralAffairs, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China 
 
Abstract:To obtain high sporulation efficiency of Bacillus subtilis strain BAB-1, the suitable culture conditions including nutrient, osmotic pressure and pH value were analyzed by using biolog phenotype microarray (PM) technology. The effects of five carbon and five nitrogen substrates on growth and sporulation of strain BAB-1 were evaluated with single factor test. The results showed that strain BAB-1 was able to metabolize 114 carbon substrates, 228 nitrogen substrates, 29 phosphorus substrates and 29 sulfur substrates.Amongthem,52carbon,66nitrogen,three sulfur and one phosphorus sources could obviously promote the growth of strain BAB-1. It had strong tolerance to osmolytes with up to 1%-10% sodium chloride, 3%-6% potassium chloride, 2%-5% sodium sulphate, 5%-20% ethylene glycol, 1%-6% sodium formate,2%-7%urea,1%-12%sodiumlactate,20-200mmol/Lsodiumphosphate,10-100mmol/L sodium nitrate and 10-100 mmol/L ammonium sulfate. Strain BAB-1 could grow well under pH between 4.5 and 10.0. Glucose and ammonium chloride were the optimum carbon and nitrogen sources for the growth of strain BAB-1, respectively, and the number of viable bacteria was significantly higher than those of other substrates at different culture durations. At 48 h, the number of viable bacteria reached 6.90×108 CFU/mL and 6.87×108 CFU/mL respectively. D-xylose, D-ribose, L-arabinose and arbutin could significantly enhance sporulation rates of strain BAB-1 at 12 h, and the sporulation rate of L-arabinose and arbutin were 61.94% and 66.92% respectively; L-proline, L-cysteine, L-glutamine and L-asparagine could prominently improve the sporulation rate of strain BAB-1 at 12 h to 36 h, and the sporulation rate reached from 27.88% to 82.37%. The results indicated that PM technology could apply to high-throughput analysis of the nutrients conducive to the growth and sporulation of B.subtilis and optimization of cultivation process.
keywords:Bacillus subtilis  phenotype microarray  sporulation  fermentation techonology
查看全文  查看/发表评论  下载PDF阅读器
您是本站第  3265629 版权所有:植物保护学报    京ICP备05006550号-2  
主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部
电话:010-62732528 电子邮件:zbxb@cau.edu.cn
技术支持:北京勤云科技发展有限公司

etiller();