• 首页 | 期刊简介 | 编委会 | 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English
环介导等温扩增技术在植物病原物检测中的应用
点此下载全文
引用本文:应淑敏,郭俭,王教瑜,李玲,张传清.环介导等温扩增技术在植物病原物检测中的应用.植物保护学报,2020,47(2):234-244
DOI:10.13802/j.cnki.zwbhxb.2020.2019063
摘要点击次数:
全文下载次数:
作者单位E-mail
应淑敏 浙江农林大学农业与食品科学学院, 临安 311300  
郭俭 浙江农林大学农业与食品科学学院, 临安 311300  
王教瑜 浙江省农业科学院植物保护与微生物研究所, 杭州 310021  
李玲 浙江农林大学农业与食品科学学院, 临安 311300 liling-06@163.com 
张传清 浙江农林大学农业与食品科学学院, 临安 311300 cqzhang@zafu.edu.cn 
中文摘要:环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种新型的由环介导的等温核酸扩增分子技术,不仅特异性强、操作简便、成本低,还能快速、高效地检测病原物,为植物病害的防控提供更精准的防治适期,从而可以减少农药的滥用。本文主要针对LAMP技术的原理、发展、优缺点、在真菌、细菌、病毒等多种植物病原物检测及在抗药性检测中的应用进行总结,并结合国内外研究进展对其应用前景进行了分析。
中文关键词:LAMP技术  检测  植物病原物
 
Application of LAMP in the detection of plant pathogens
Author NameAffiliationE-mail
YING Shumin College of Agricultural and Food Sciences, Zhejiang Agriculture and Forestry University, Lin'an 311300, Zhejiang Province, China  
GUO Jian College of Agricultural and Food Sciences, Zhejiang Agriculture and Forestry University, Lin'an 311300, Zhejiang Province, China  
WANG Jiaoyu Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China  
LI Ling College of Agricultural and Food Sciences, Zhejiang Agriculture and Forestry University, Lin'an 311300, Zhejiang Province, China liling-06@163.com 
ZHANG Chuanqing College of Agricultural and Food Sciences, Zhejiang Agriculture and Forestry University, Lin'an 311300, Zhejiang Province, China cqzhang@zafu.edu.cn 
Abstract:Loop-mediated isothermal amplification (LAMP) technology is a new type of loop-mediated isothermal nucleic acid amplification molecular technology, which has significant advantages such as strong specificity, easy operation and low cost, and was used to fast, high efficiently dectect patheogens. LAMP technology provides more accurate control period for the effective prevention and control of plant diseases, so as to reduce the abuse of pesticide. This paper mainly summarizes the principle, development, advantages and disadvantages of LAMP technology, and its application in the detection of various plant pathogens such as fungi, bacteria, and viruses, as well as the detection for pesticide resistance, and analyzes its application prospects in accordance with domestic and foreign research progress.
keywords:loop-mediated isothermal amplification (LAMP)  detection  plant pathogens
查看全文  查看/发表评论  下载PDF阅读器
您是本站第  3265623 版权所有:植物保护学报    京ICP备05006550号-2  
主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部
电话:010-62732528 电子邮件:zbxb@cau.edu.cn
技术支持:北京勤云科技发展有限公司

etiller();