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功能基因组技术在昆虫抗药性研究中的应用展望
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引用本文:陈皓,彭威,韩宝瑜.功能基因组技术在昆虫抗药性研究中的应用展望.植物保护学报,2020,47(2):223-233
DOI:10.13802/j.cnki.zwbhxb.2020.2019100
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陈皓 中国计量大学生命科学学院, 浙江省生物计量及检验检疫技术重点实验室, 杭州 310018  
彭威 中国计量大学生命科学学院, 浙江省生物计量及检验检疫技术重点实验室, 杭州 310018  
韩宝瑜 中国计量大学生命科学学院, 浙江省生物计量及检验检疫技术重点实验室, 杭州 310018 hanby15@163.com 
中文摘要:目前化学防治仍是植物保护的重要手段,化学杀虫剂通过参与昆虫自然选择,诱导其产生可遗传的基因突变,从而导致昆虫抗药性的增加,近年来随着杀虫剂的广泛使用,抗药性已成为植物保护领域亟需解决的重大问题。利用分子生物学手段对由基因变异引起的昆虫抗药性进行解析已成为可能,在现今大多数昆虫抗药性研究中,功能基因组技术已被广泛用于候选基因调控抗药性机制的研究。本文介绍了双元基因表达技术——GAL4/UAS技术、基因干扰技术——RNAi技术和基因编辑技术——CRISPR/Cas9技术这3种有助于阐明昆虫抗药性分子机制的功能基因组技术,综述了这3种功能基因组技术在近几年昆虫抗药性研究中的应用实例,讨论了其目前的发展状况及其优势和局限性,以期在完善的功能基因组技术支持下为昆虫抗药性研究取得更多突破性进展。
中文关键词:昆虫抗药性  功能基因组技术  GAL4/UAS技术  RNA干扰技术  CRISPR/Cas9技术
 
Application prospects of functional genome analysis technologies in insecticide resistance researches
Author NameAffiliationE-mail
CHEN Hao Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang Province, China  
PENG Wei Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang Province, China  
HAN Baoyu Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang Province, China hanby15@163.com 
Abstract:At present, chemical control is still the significant approach for plant protection. Chemical insecticides participate in natural selection of insects by inducing hereditary genetic mutations, resulting in an increase of insecticide resistance. With the wide applications of insecticides in recent years, the resistance has become a crucial problem that needs to be solved in the field of plant protection. It is now possible to use methods in molecular biology to analyze insecticide resistance caused by genetic mutations. Functional genome analysis technologies have been widely used in the study on the mechanism of candidate genes in regulating insecticide resistance. This article introduced three functional genomic techniques that helped clarify the molecular mechanisms of insecticide resistance:the binary gene expression technology-GAL4/UAS technology, the gene interference technology-RNAi technology and the gene editing technology-CRISPR/Cas9 technology. Meanwhile, this article reviewed the application examples for the three functional genomic techniques in insecticide resistance research in recent years, discussed the current development and their advantages and limitations, with a view to make more breakthrough progresses in the insecticide resistance research based on the comprehensive functional genomic technologies.
keywords:insect resistance  functional genome analysis technology  GAL4/UAS technology  RNA interference technology  CRISPR/Cas9 technology
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