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大樱桃病毒潜在过渡寄主和昆虫介体的检测
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引用本文:张雅雯,窦宝存,陈珍珍,曹欣然,原雪峰.大樱桃病毒潜在过渡寄主和昆虫介体的检测.植物保护学报,2020,47(1):161-168
DOI:10.13802/j.cnki.zwbhxb.2020.2019059
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作者单位E-mail
张雅雯 山东农业大学植物保护学院, 泰安 271000  
窦宝存 山东农业大学植物保护学院, 泰安 271000  
陈珍珍 山东农业大学植物保护学院, 泰安 271000  
曹欣然 青岛农业大学植物医学院, 山东 青岛 266109 xinran1001@163.com 
原雪峰 山东农业大学植物保护学院, 泰安 271000 snowpeak77@163.com 
中文摘要:为分析大樱桃病毒潜在的过渡寄主和昆虫介体,利用反转录PCR(reverse transcription-PCR,RT-PCR)对采集自大樱桃园内的杂草、昆虫及园区周边的常绿植物进行大樱桃8种病毒的检测,通过蛋白质印迹法(Western blot)对RT-PCR检测结果进行验证,并经过BLAST比对和系统发育树构建分析大樱桃病毒分离株的亲缘关系。结果显示,分别从大樱桃园内4种杂草商陆Phytolacca acinosa、鸭趾草Commelina communis、马齿苋Portulaca oleracea和一年蓬Erigeron annuus,园区周边3种植物山枣Ziziphus montana、石楠Photinia serrulata和板栗Castanea mollissima,2种害虫绿盲蝽Apolygus lucorum和赤须盲蝽Trigonotylus ruficornis中检测到黄瓜花叶病毒(cucumber mosaic virus,CMV);而未检测到其它7种大樱桃病毒;且Western blot验证结果显示RT-PCR检测结果可靠。4种杂草、3种周边植物和2种昆虫的CMV RNA3序列均与大樱桃CMV分离株YT-CH(登录号KY646104)的RNA3序列相似度较高,为97.08%~99.54%;系统发育树显示,商陆CMV-PA分离株与大樱桃分离株YT-CH的亲缘性较高,赤须盲蝽分离株CMV-CX与马齿苋分离株CMV-PO、山枣分离株CMV-ZM及石楠分离株CMV-PS的亲缘性较高。表明上述7种植物和2种盲蝽是大樱桃病毒的潜在过渡寄主和昆虫介体,在大樱桃病毒病防治中应给予一定的关注。
中文关键词:大樱桃病毒  昆虫介体  过渡寄主  黄瓜花叶病毒(CMV)
 
Detection of potential transitional hosts and insect vectors of cherry viruses
Author NameAffiliationE-mail
ZHANG Yawen College of Plant Protection, Shandong Agricultural University, Tai'an 271000, Shandong Province, China  
DOU Baocun College of Plant Protection, Shandong Agricultural University, Tai'an 271000, Shandong Province, China  
CHEN Zhenzhen College of Plant Protection, Shandong Agricultural University, Tai'an 271000, Shandong Province, China  
CAO Xinran College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China xinran1001@163.com 
YUAN Xuefeng College of Plant Protection, Shandong Agricultural University, Tai'an 271000, Shandong Province, China snowpeak77@163.com 
Abstract:To analyze the potential transition hosts and vectors for cherry viruses, RT-PCR and Western blot were used to detect the existence of eight types of cherry viruses from weeds and insects in cherry orchards, and peripheral woodyplants of cherry orchards. The results showed that cucumber mosaic virus (CMV) could be detected from four species of weeds (Phytolacca acinosa, Commelina communis, Portulaca oleracea and Erigeron annuus), three species of woody plants (Ziziphus montana, Photinia serrulata and Castanea mollissima), and two species of agricultural pests (Apolygus lucorum and Trigonotylus ruficornis), while other seven types of cherry viruses failed to be detected. The similarity of CMV RNA3 sequences between the four weeds, three plants, two insect species and cherry CMV isolates YT-CH (KY646104) was high, up to 97.08%-99.54%. The results of phylogenetic analysis showed that the affinity between CMV-PA isolates of P. acinosa and cherry isolates YT-CH was high, and the affinity between CMV-CX isolate of T. ruficornis and CMV-PO isolate of P. oleracea, CMV-ZM isolate of Z. montana, CMV-PS isolate of P. serrulata were also high. These plants and agricultural pests were potential transitional hosts and vectors of CMV, providing basic data for control of the disease caused by CMV.
keywords:cherry virus  insect vector  transitional host  cucumber mosaic virus (CMV)
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