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西瓜噬酸菌hflX基因功能分析
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引用本文:乔培,刘博,杨玉文,郭彦彤,关巍,赵廷昌.西瓜噬酸菌hflX基因功能分析.植物保护学报,2020,47(1):150-160
DOI:10.13802/j.cnki.zwbhxb.2020.2019046
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作者单位E-mail
乔培 吉林农业大学农学院, 长春 130118
中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 
 
刘博 吉林农业大学农学院, 长春 130118  
杨玉文 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193  
郭彦彤 吉林农业大学农学院, 长春 130118  
关巍 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193  
赵廷昌 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 zhaotgcg@163.com 
中文摘要:为明确GTP结合蛋白基因hflX在西瓜噬酸菌Acidovorax citrulli中的功能尤其是对该菌致病力的影响,以西瓜噬酸菌Aac5菌株为材料获得hflX基因的缺失突变菌株及互补菌株,通过对致病力等表型以及相关基因表达量的测定初步探究西瓜噬酸菌hflX基因的功能。结果显示,同野生型菌株Aac5相比,缺失突变菌株的致病力显著下降,III型分泌系统基因hrpGhrpE的表达量显著下调,仅为野生型菌株表达量的54.99%和27.39%;hflX基因的缺失并未影响其引起烟草过敏性坏死反应的能力;但缺失突变菌株的运动能力显著下降,鞭毛基因fliRfliC的表达量显著下调,仅分别为野生型菌株的37.04%和29.68%。此外,缺失突变菌株的生物膜形成能力相较于野生型菌株显著增强了26.32%,同时,其体外生长能力也有所增强。表明hflX基因可能通过调控III型分泌系统基因hrpGhrpE、鞭毛基因fliRfliC的表达以及运动能力来影响西瓜噬酸菌Aac5菌株的致病力。
中文关键词:西瓜噬酸菌  hflX  致病力  功能分析
 
Functional analysis of fruit blotch pathogen hflX in Acidovorax citrulli
Author NameAffiliationE-mail
QIAO Pei College of Agriculture, Jilin Agricultural University, Changchun 130118, Jilin Province, China
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 
 
LIU Bo College of Agriculture, Jilin Agricultural University, Changchun 130118, Jilin Province, China  
YANG Yuwen State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China  
GUO Yantong College of Agriculture, Jilin Agricultural University, Changchun 130118, Jilin Province, China  
GUAN Wei State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China  
ZHAO Tingchang State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China zhaotgcg@163.com 
Abstract:In order to clarify the function of GTP-binding protein gene hflX in fruit blotch pathogen Acidovorax citrulli and its effect on pathogenicity, A. citrulli Aac5 strain was used as wild-type strain, resulting in a hflX-deletion mutant and its complementary strain. Compared with the wild-type strain Aac5, hflX mutant showed significantly reduced virulence, and the expression levels of type III secretion system genes hrpG and hrpE decreased significantly, only 54.99% and 27.39% of the wild-type strain. Deletion of hflX did not influence the bacterial ability to trigger hypersensitive response on non-host Nicotiana tabacum var. Samsun; the swimming motility significantly reduced, the expression levels of flagellar genes fliR and fliC decreased significantly, only 37.04% and 29.68% of the wild-type strain. In addition, the biofilm formation ability of hflX mutant strain was 26.32% higher than that of the wild strain, and enhanced significantly. The growth ability in vitro of hflX mutant strain had also been enhanced. The results indicated that hflX might regulate virulence of A. citrulli Aac5 strain by expression of type III secretion system genes hrpG and hrpE, flagellar genes fliR and fliC, and swimming motility.
keywords:Acidovorax citrulli  hflX  virulence  functional analysis
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