• 首页 | 期刊简介 | 编委会 | 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English
黑龙江省稻瘟病菌育性及其交配型分析
点此下载全文
引用本文:张晓玉,张亚玲,靳学慧,周弋力,孟峰,武菁菁.黑龙江省稻瘟病菌育性及其交配型分析.植物保护学报,2020,47(1):93-100
DOI:10.13802/j.cnki.zwbhxb.2020.2019058
摘要点击次数:
全文下载次数:
作者单位E-mail
张晓玉 黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319  
张亚玲 黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319  
靳学慧 黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319 Jxh2686@163.com 
周弋力 黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319  
孟峰 黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319  
武菁菁 黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319  
中文摘要:为明确黑龙江省采集自不同年份、不同地区的稻瘟病菌Magnaporthe oryzae的育性能力和交配型分布,采用2株标准菌株GUY11(MAT1-2)和KA3(MAT1-1)对2016-2017年黑龙江省西部、东部、中部3个地区经单孢分离的241株稻瘟病菌进行育性测定,并利用PCR技术对其交配型进行检测。结果表明,黑龙江省西部、东部、中部的241株稻瘟病菌中可育性菌株比例为11.62%,其中雌性菌株、雄性菌株、两性菌株分别占1.66%、4.56%和1.25%,不能判断其性别的未知菌株占4.15%。采集自不同地区、不同年份的稻瘟病菌可育性差异均较大,西部、东部、中部地区可育性菌株出现频率分别为13.25%、7.27%和12.62%;2016年采集的稻瘟病菌可育性较高,可育性菌株出现频率为25.30%。黑龙江省稻瘟病菌群体中同时存在MAT1-1MAT1-2两种交配型,主要以交配型MAT1-1占优势,出现频率为58.92%,交配型为MAT1-2的菌株出现频率为8.30%。不同地区稻瘟病菌的交配型亦有差异,交配型为MAT1-1的菌株在黑龙江省东部地区出现频率最高,为72.73%,在中部、西部地区的出现频率次之,分别为61.17%和46.99%。表明黑龙江省水稻种植区的稻瘟病菌同时存在2种交配型菌株,其交配型存在丰富的多态性,但其可育性及交配型分布不均衡。
中文关键词:稻瘟病菌  有性生殖  交配型  育性  PCR
 
Analysis of the fertility and mating type of rice blast fungus Magnaporthe oryzae in Heilongjiang Province
Author NameAffiliationE-mail
ZHANG Xiaoyu Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China  
ZHANG Yaling Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China  
JIN Xuehui Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China Jxh2686@163.com 
ZHOU Yili Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China  
MENG Feng Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China  
WU Jingjing Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China  
Abstract:In order to clarify the distribution of fertility and mating types of Magnaporthe oryzae collected from different regions of Heilongjiang Province and in different years, 241 isolates of M. grisea collected from western, eastern and central regions of Heilongjiang Province from 2016 to 2017 were tested for their mating types and fertility with two standard test isolates GUY11 (MAT1-2) and KA3 (MAT1-1) and their mating types were detected by PCR. The results showed that the percentage of fertile isolates in 241 isolates of M. oryzae was 11.62%, of which female isolates, male isolates and amphipathic isolates accounted for 1.66%, 4.56% and 1.25%, respectively, and unknown isolates that could not judge their sex accounted for 4.15%. The fertility of rice blast fungus collected from different regions and in different years varied greatly. The frequency of fertility isolates in western, eastern and central regions was 13.25%, 7.27% and 12.62%, respectively. The frequency of fertility isolates of M. oryzae collected in 2016 was higher (25.30%). There were two mating types of MAT1-1 and MAT1-2 existing simultaneously in Heilongjiang Province:mating type MAT1-1 was dominant, the frequency was 58.92%, and the frequency of mating type MAT1-2 was 8.30%. The mating types of M. oryzae in different regions also differed greatly. The isolate with mating type MAT1-1 occurred the most frequently in the eastern part of Heilongjiang Province, the frequency was 72.73%, followed by the frequencies of the central and western regions, accounting for 61.17% and 46.99%, respectively. The results indicated that there were two mating isolates in the rice-growing areas of Heilongjiang Province, and the mating type was rich in polymorphism, but the distribution of fertility and mating type was imbalanced.
keywords:Magnaporthe oryzae  sexual reproduction  mating type  fertility  PCR
查看全文  查看/发表评论  下载PDF阅读器
您是本站第  2940713 版权所有:植物保护学报    京ICP备05006550号-2  
主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部
电话:010-62732528 电子邮件:zbxb@cau.edu.cn
技术支持:北京勤云科技发展有限公司

etiller();