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载体中蓟马聚集信息素组分的释放速率测定及其储藏温度筛选
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引用本文:李晓维,孙冉冉,章金明,张治军,张立猛,吕要斌.载体中蓟马聚集信息素组分的释放速率测定及其储藏温度筛选.植物保护学报,2020,47(1):26-34
DOI:10.13802/j.cnki.zwbhxb.2020.2018203
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作者单位E-mail
李晓维 浙江省农业科学院植物保护与微生物研究所, 农产品质量安全危害因子与风险防控国家重点实验室(筹), 杭州 310021  
孙冉冉 浙江省农业科学院植物保护与微生物研究所, 农产品质量安全危害因子与风险防控国家重点实验室(筹), 杭州 310021
南京农业大学植物保护学院, 南京 210095 
 
章金明 浙江省农业科学院植物保护与微生物研究所, 农产品质量安全危害因子与风险防控国家重点实验室(筹), 杭州 310021  
张治军 浙江省农业科学院植物保护与微生物研究所, 农产品质量安全危害因子与风险防控国家重点实验室(筹), 杭州 310021  
张立猛 云南省烟草公司玉溪市公司, 玉溪 653100  
吕要斌 浙江省农业科学院植物保护与微生物研究所, 农产品质量安全危害因子与风险防控国家重点实验室(筹), 杭州 310021
南京农业大学植物保护学院, 南京 210095 
luybcn@163.com 
中文摘要:为明确蓟马聚集信息素组分在2种常用载体中的释放规律,应用固相微萃取(SPME)和气相色谱-质谱联用技术测定不同聚集信息素剂量(1、10、100 μg)和萃取时间(30、60、120 min)下SPME萃取头对载体中3种蓟马聚集信息素组分的萃取效率,筛选最佳萃取条件,并分析储藏温度对载体中蓟马聚集信息素组分释放速率的影响。结果表明,在橡胶塞和PVC管2种载体中,相同聚集信息素剂量下,与萃取30 min处理相比,萃取60、120 min时SPME萃取头对载体释放的3种聚集信息素组分的萃取效率或无显著差异,或随萃取时间增加而呈现降低趋势;相同萃取时间下,SPME萃取头对载体释放的3种聚集信息素组分的萃取效率均随剂量增加而显著增大。因此,测定载体中聚集信息素释放速率的最佳条件为萃取时间30 min、剂量100 μg。室温25℃储藏条件下,橡胶塞和PVC管中3种聚集信息素组分的释放速率均在存储2 d时最高,之后随存放时间的延长而逐渐降低;-20℃储藏条件下,载体中3种聚集信息素组分的释放速率随着存放时间的延长相对稳定,56 d内释放速率均处于较高水平,且显著高于室温下储藏载体中聚集信息素组分的释放速率。表明-20℃储藏可有效减少载体中聚集信息素组分的损耗,即-20℃是聚集信息素载体储藏的适宜温度。
中文关键词:聚集信息素  缓释载体  固相微萃取(SPME)  释放速率  储藏温度
 
The release rates of thrips aggregation pheromones from two dispensers and the selection of storage temperature
Author NameAffiliationE-mail
LI Xiaowei State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China  
SUN Ranran State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China 
 
ZHANG Jinming State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China  
ZHANG Zhijun State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China  
ZHANG Limeng Yuxi Tobacco Company, Yuxi 653100, Yunnan Province, China  
Lü Yaobin State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China 
luybcn@163.com 
Abstract:To understand the release dynamics of the three thrips aggregation pheromone compounds, the extraction efficiency under different pheromone doses and solid phase microextraction (SPME) extraction time was investigated for the three thrips aggregation pheromone compounds from two kinds of dispensers by using SPME and gas chromatography-mass spectrometer (GC-MS). The effect of storage temperature on the release rates of aggregation pheromone compounds from dispensers was also investigated. The results showed that, for both dispensers, the SPME fiber extraction efficiency of the three aggregation pheromone compounds extracted for 60 min or 120 min was either not significantly different, or significantly lower compared with extraction for 30 min. The doses had significant effect on SPME extraction efficiency of the three aggregation pheromone compounds:the higher the dose, the higher the extraction efficiency. Consequently, the optimal experimental conditions for release rate measurement were 30 min of SPME extraction and 100 μg of pheromone compounds. When stored at room temperature (25℃), the release rates of the three aggregation pheromone compounds from two dispensers were the highest after stored for 2 d; the release rates then decreased with the storage time. By contrast, when stored at -20℃, the release rates of the three aggregation pheromone compounds from two dispensers either stayed stable or fluctuated along the storage time, but for 56 d, the release rates were consistently higher than those stored at 25℃. The results indicated that storing at -20℃ could greatly reduce the loss of pheromone compounds during storage. Consequently, -20℃ is recommended as the storage temperature for aggregation pheromone dispensers.
keywords:aggregation pheromone  dispenser  solid phase microextraction (SPME)  release rate  storage temperature
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