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寄主诱导的基因沉默技术研究和应用进展
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引用本文:肖瑶,王教瑜,李玲,王艳丽,张传清,孙国昌.寄主诱导的基因沉默技术研究和应用进展.植物保护学报,2020,47(1):11-17
DOI:10.13802/j.cnki.zwbhxb.2020.2019069
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作者单位E-mail
肖瑶 浙江农林大学农业与食品科学学院, 临安 311300
浙江省农业科学院植物保护与微生物研究所, 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 杭州 310021 
 
王教瑜 浙江省农业科学院植物保护与微生物研究所, 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 杭州 310021 wangjiaoyu78@sina.com 
李玲 浙江农林大学农业与食品科学学院, 临安 311300  
王艳丽 浙江省农业科学院植物保护与微生物研究所, 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 杭州 310021  
张传清 浙江农林大学农业与食品科学学院, 临安 311300  
孙国昌 浙江省农业科学院植物保护与微生物研究所, 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 杭州 310021 sungc01@sina.com 
中文摘要:病原真菌严重威胁着农作物的产量和品质,提高作物抗性是病原真菌病害防控的重要措施。寄主诱导的基因沉默(host induced gene silencing,HIGS)技术是在RNA干扰的基础上发展而来,以病原真菌生长发育和侵染过程中的关键基因为靶标,通过在寄主植物中表达这些基因的干扰RNA从而抑制病原真菌中靶标基因的表达,达到抵制病原真菌扩展,提高寄主植物抗病性的目的。近年来,HIGS技术被用于防控多种由病原真菌引起的病害,并取得了明显成效,为植物抗病资源的开发及应用提供了新途径。本文综述了HIGS技术的原理、技术路线、操作方法和国内外的主要研究进展,总结了操作中需要注意的事项,并对该技术的发展趋势和应用前景进行了展望。
中文关键词:病原真菌  RNA干扰  寄主诱导的基因沉默
 
Advances in the researches and applications of host-induced gene silencing technology
Author NameAffiliationE-mail
XIAO Yao School of Agricultural and Food Sciences, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang Province, China
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China 
 
WANG Jiaoyu State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China wangjiaoyu78@sina.com 
LI Ling School of Agricultural and Food Sciences, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang Province, China  
WANG Yanli State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China  
ZHANG Chuanqing School of Agricultural and Food Sciences, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang Province, China  
SUN Guochang State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China sungc01@sina.com 
Abstract:Pathogenic fungi seriously threaten the output and quality of crops, and improving crop resistance is an important measure for the prevention and control of pathogenic fungi. Host-induced gene silencing (HIGS) technology was developed based on RNA interference, which targeted the key genes in the growth and development and the infection process of pathogenic fungi by expressing the interfering RNAs in host plants to inhibit the expression of target genes in pathogenic fungi and improve the disease resistance in the host plants. In recent years, HIGS technology has been used to prevent and control a variety of diseases caused by pathogenic fungi and achieved significant results, providing a new way for the development and application of plant disease-resistant resources. This review summarized the principles, technical routes, operation methods and main progresses in HIGS technology at home and abroad, summarized the issues that should be paid attention to during operation, and predicted the prospects in the development and application of the technology.
keywords:plant fungal disease  RNA interference  host-induced gene silencing
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