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威百亩熏蒸对土壤硝化作用及amoA型硝化细菌群落结构的影响
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引用本文:燕平梅,李娜,赵文婧,陈燕飞,乔宏萍.威百亩熏蒸对土壤硝化作用及amoA型硝化细菌群落结构的影响.植物保护学报,2019,46(6):1357-1363
DOI:10.13802/j.cnki.zwbhxb.2019.2018191
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作者单位E-mail
燕平梅 太原师范学院生物系, 土壤消毒活化绿色产业技术创新战略联盟, 太原 030619 yanpingmei1968@163.com 
李娜 太原师范学院生物系, 土壤消毒活化绿色产业技术创新战略联盟, 太原 030619  
赵文婧 太原师范学院生物系, 土壤消毒活化绿色产业技术创新战略联盟, 太原 030619  
陈燕飞 太原师范学院生物系, 土壤消毒活化绿色产业技术创新战略联盟, 太原 030619  
乔宏萍 太原师范学院生物系, 土壤消毒活化绿色产业技术创新战略联盟, 太原 030619  
中文摘要:为探究威百亩对土壤硝化作用的影响及作用机制,通过理化分析和变性梯度凝胶电泳方法研究浓度为20、100和500 mg/kg威百亩熏蒸处理对土壤硝化作用及amoA型硝化细菌群落结构的影响。结果表明,威百亩熏蒸处理0、14、28 d后,3种浓度处理的土壤硝化率与对照差异不显著;处理56 d后,20、100和500 mg/kg浓度处理的土壤硝化率分别为81.15%、76.66%和94.08%,前两者与对照差异不显著,后者与对照差异显著;而处理84 d后,3种浓度处理的土壤硝化率与对照差异均不显著。与对照相比,3个浓度威百亩熏蒸处理不同时间后土壤amoA型硝化细菌群落的均匀度指数差异不显著。熏蒸处理14 d后,浓度20、100 mg/kg威百亩处理的土壤amoA型硝化细菌群落的香农多样性指数和丰富度指数分别为1.00、10.97和0.84、7.79,与对照的0.92和9.78均无显著差异;而浓度500 mg/kg威百亩处理的香农多样性指数和丰富度指数分别为0.59、5.77,显著低于对照。表明威百亩对土壤硝化强度的影响较amoA型硝化细菌群落延迟,说明土壤中硝化作用的强弱并非完全决定于土壤amoA型硝化细菌的作用。
中文关键词:威百亩  土壤熏蒸剂  硝化作用  氨单加氧酶  变性梯度凝胶电泳
 
Effects of metham sodium fumigation on soil nitrification and the community structure of amoA nitrification bacteria
Author NameAffiliationE-mail
Yan Pingmei Green Industry Technology Innovation Strategic Alliance of Soil Disinfestation and Activation, Department of Biology, Taiyuan Normal University, Taiyuan 030619, Shanxi Province, China yanpingmei1968@163.com 
Li Na Green Industry Technology Innovation Strategic Alliance of Soil Disinfestation and Activation, Department of Biology, Taiyuan Normal University, Taiyuan 030619, Shanxi Province, China  
Zhao Wenjing Green Industry Technology Innovation Strategic Alliance of Soil Disinfestation and Activation, Department of Biology, Taiyuan Normal University, Taiyuan 030619, Shanxi Province, China  
Chen Yanfei Green Industry Technology Innovation Strategic Alliance of Soil Disinfestation and Activation, Department of Biology, Taiyuan Normal University, Taiyuan 030619, Shanxi Province, China  
Qiao Hongping Green Industry Technology Innovation Strategic Alliance of Soil Disinfestation and Activation, Department of Biology, Taiyuan Normal University, Taiyuan 030619, Shanxi Province, China  
Abstract:To explore the effect and mechanism of metham on soil nitrification, physicochemical analysis and denaturing gradient gel electrophoresis were used to analyze the effects of 20, 100 and 500 mg/kg of fumarate on soil nitrification and amoA type denitrifying bacteria community structure. The results showed that after 0, 14 and 28 days of fumigation treatment, the soil nitrification rates of 20, 100 and 500 mg/kg treatments were not significantly different from those of the control. After 56 days of treatment, the soil nitrification rate of the 20, 100 and 500 mg/kg treatments were 81.15%, 76.66% and 94.08%, respectively. There was no significant difference between the two treatments and that of the control, but the significant difference appered latter. After 84 days of treatment, there was no significant difference in soil nitrification between 20, 100 and 500 mg/kg treatments and the control group. Compared with the control group, there was no significant difference in evenness index of amoA type nitrifying bacteria community after 0, 14, 28, 56 and 84 days of fumigation with three concentrations of metham. After 14 days of fumigation treatment, the diversity index and richness index of amoA-type nitrifying bacteria community in soil treated with 20, 100 mg/kg concentrations were 1.00, 10.97 and 0.84, 7.79, respectively, which had no significant with those of control (0.92 and 9.78); the diversity index and richness index of treatment with 500 mg/kg concentration were 0.59 and 5.77, were remarkable lower than those of control. The effect of metham on soil nitrification intensity was delayed compared with that of amoA type nitrifying bacteria community structure, which indicated that the strength of nitrification in soil does not depend entirely on the number of nitrifying bacteria in the soil.
keywords:metham  soil fumigant  nitrification  amoA  denaturing gradient gel electrophoresis(DGGE)
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