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50% TraT2A水分散粒剂研制及其增效剂筛选
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引用本文:李嘉明,梁巧兰,魏列新,姜玉玲,吴琼.50% TraT2A水分散粒剂研制及其增效剂筛选.植物保护学报,2019,46(6):1324-1334
DOI:10.13802/j.cnki.zwbhxb.2019.2018211
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作者单位E-mail
李嘉明 甘肃农业大学植物保护学院, 甘肃省农作物病虫害生物防治工程实验室, 兰州 730070  
梁巧兰 甘肃农业大学植物保护学院, 甘肃省农作物病虫害生物防治工程实验室, 兰州 730070 liangql@gsau.edu.cn 
魏列新 甘肃农业大学植物保护学院, 甘肃省农作物病虫害生物防治工程实验室, 兰州 730070  
姜玉玲 甘肃农业大学植物保护学院, 甘肃省农作物病虫害生物防治工程实验室, 兰州 730070  
吴琼 甘肃农业大学植物保护学院, 甘肃省农作物病虫害生物防治工程实验室, 兰州 730070  
中文摘要:为提高深绿木霉Trichoderma atroviride T2菌株发酵液蛋白提取物TraT2A的稳定性及诱导抗病活性,通过筛选合适助剂、优化配方后,采用挤压造粒法制备50% TraT2A水分散粒剂,对其进行质量标准和活性评价;并通过增效剂筛选研制该制剂的复配增效剂。结果显示,以优化后的50% TraT2A水分散粒剂配方(质量分数)为50.00% TraT2A、2.00%十二烷基苯磺酸钠、8.00%聚羧酸盐、0.50%聚乙烯吡咯烷酮、4.50%硫酸铵、1.00%抗坏血酸、34.00%煅烧高岭土制得的制剂,其有效成分质量分数为48.60%,外观为白色颗粒状固体,颗粒破碎率为4.43%,水分含量为2.10%,崩解时间为57.13 s,悬浮率为83.04%,细度(75 μm湿筛)为100.00%,粒度(100 μm标准试验筛)为95.00%,pH为7.90,持久起泡性为250 mL试管中泡沫高度12.25 mL,经热贮、冷贮后该制剂外观及各项指标均无明显变化,有效成分分解率分别为3.60%和2.00%,均达到国家质量控制标准。当50% TraT2A水分散粒剂浓度分别为40、20 mg/mL时,对灰霉菌Botrytis cinerea的抑制率及对百合抗灰霉病的诱抗效果达到最高,分别为61.79%和65.77%;当增效剂0.50 mg/mL JFC和0.20 mg/mL杰效利按体积比3:7混合时增效最显著,诱抗效果达74.20%,增效为12.82%。表明50% TraT2A水分散粒剂质量合格,且该制剂高浓度时对灰霉菌有一定抑制作用,低浓度时有较高的诱导抗病作用;所得复配增效剂对该制剂的诱导抗病作用增效明显。
中文关键词:TraT2A  水分散粒剂  灰霉菌  抑菌活性  诱抗效果  增效作用
 
Development and synergist screening of 50% TraT2A water-dispersible granules
Author NameAffiliationE-mail
Li Jiaming Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Liang Qiaolan Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China liangql@gsau.edu.cn 
Wei Liexin Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Jiang Yuling Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Wu Qiong Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Abstract:In order to improve the stability of the protein extract TraT2A from the fermentation broth of Trichoderma atroviride T2 strain and its activity in inducing disease resistance, the 50% TraT2A waterdispersible granules was prepared through extrusion granulation, auxiliary agent screening and formula optimization, and its qualitative criteria and activity as the effective constituent were evaluated. The synergistic agent was screened to develop a compound synergist for this preparation. The results showed that the optimized 50% TraT2A water-dispersible granules, prepared by the formula (mass fraction) of 50.00% TraT2A, 2.00% sodium dodecyl benzene sulfonate (SDBS), 8.00% 5040 (polycarboxylate), 0.50% polyvinylpyrrolidone (PVP), 4.50% ammonium sulfate, 1.00% ascorbic acid and 34.00% calcined kaolin, had an effective component mass fraction of 48.60% with white granular solid appearance, a particle breaking rate of 4.43%, a moisture content of 2.10%, a disintegrating time of 57.13 s and a suspension rate of 83.04%. The fineness (including 75 μm wet-screened) of the granules was 100.00%; the particle size (including 100 μm standard test sieve) was 95.00% and the pH was 7.90. The foaming test result was 12.25 mL in 250 mL test tube. After thermal storage and cold storage, these parameters and the appearance of the preparations were not significantly changed. The decomposition rate of the effective component was 3.60% during the thermal storage and 2.00% during the cold storage. All quality criteria met the national quality control standards. The inhibition activity and the inductive resistance against Botrytis cinerea was the highest at the concentration of 40 mg/mL and 20 mg/mL, up to 61.79% and 65.77%, respectively. When the synergists JFC (0.5 mg/mL) and Jiexiaoli (0.2 mg/mL) were mixed at the volume ratio of 3:7, the synergistic effect was the best; the induction of B. cinerea disease resistance reached 74.20% and the synergistic effect was 12.82%. All results indicated that the quality of the 50% TraT2A water-dispersible granules was satisfactory with an effective inhibition activity against B. cinerea at a high concentration, a relatively high induction of disease resistance at a low concentration and an obvious synergistic effect of the synergistic agent on disease resistance induction.
keywords:TraT2A  water-dispersible granule  Botrytis cinerea  inhibition activity  induced resistance effect  synergism
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