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糜子细菌性条斑病病原菌鉴定及其对13种杀菌剂的敏感性
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引用本文:白雪,陈悦,白庆荣,谷月,孙蕾,赵廷昌.糜子细菌性条斑病病原菌鉴定及其对13种杀菌剂的敏感性.植物保护学报,2019,46(6):1233-1242
DOI:10.13802/j.cnki.zwbhxb.2019.2018144
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作者单位E-mail
白雪 吉林农业大学植物保护学院, 长春 130118
吉林省农业科学院植物保护研究所, 公主岭 136100 
 
陈悦 吉林农业大学植物保护学院, 长春 130118  
白庆荣 吉林农业大学植物保护学院, 长春 130118 bbbqqqrrr@163.com 
谷月 吉林农业大学植物保护学院, 长春 130118  
孙蕾 吉林农业大学植物保护学院, 长春 130118  
赵廷昌 中国农业科学院植物保护研究所, 北京 100193 zhaotgcg@163.com 
中文摘要:为有效防治糜子细菌性条斑病,采用平板划线分离法对2016年自黑龙江省齐齐哈尔市采集的罹细菌性条斑病的糜子植株进行病原菌分离与纯化,依据柯赫氏法则对其致病性进行测定;采用Biolog自动微生物鉴定系统、16S rDNA和16S-23S rDNA间隔区序列分析及病原菌的生理生化反应对病原菌进行鉴定;并测定该病原菌对13种常用杀菌剂的敏感性。结果显示,从糜子叶片和叶鞘病斑中共分离纯化获得10株培养性状一致的菌株,经柯赫氏法则验证均为细菌性条斑病病原菌。经Biolog鉴定,3株代表菌株与标准菌株燕麦噬酸菌Acidovorax avenae的相似值分别为0.716、0.705、0.723,序列分析显示,这3株代表菌株与燕麦噬酸菌燕麦亚种A.avenae subsp.avenae的一致性为99%以上;该病原菌的革兰氏染色反应及碳源利用等生理生化反应与对照菌株燕麦噬酸菌ICMP3183表现一致。因此,确定糜子细菌性条斑病病原菌为燕麦噬酸菌。糜子细菌性条斑病病原菌代表菌株MZ121101对80%乙蒜素乳油、30%乙蒜素乳油及0.3%四霉素水剂的敏感性较高,抑制中浓度EC50介于389.31~939.94 μg/mL之间,这2种药剂可作为防治糜子细菌性条斑病的首选药剂。
中文关键词:糜子  细菌性条斑病  燕麦噬酸菌  病原菌鉴定  杀菌剂  敏感性
 
Identification of pathogen causing bacterial stripe of millet and its susceptibility to 13 bactericides
Author NameAffiliationE-mail
Bai Xue College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China
Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China 
 
Chen Yue College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China  
Bai Qingrong College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China bbbqqqrrr@163.com 
Gu Yue College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China  
Sun Lei College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China  
Zhao Tingchang Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China zhaotgcg@163.com 
Abstract:To control millet bacterial stripe, the method of streaking plate was used to isolate pathogen from the diseased samples that were collected from Qiqihaer City, Heilongjiang Province in 2016. The pathogenicity of the isolates was tested to fulfill by the Koch's postulates. Biolog systematic, sequences analyses of 16S rDNA and 16S-23S rDNA, biochemical and physiological reactions were used in pathogen identification. The sensitivity of representative strain to 13 bactericides was also determined. Ten isolates were obtained from the diseased leaves and sheaths with similar morphology on medium, and were verified as the pathogens of the bacterial stripe of millet. The results of Biolog test showed three representative strains were similar with Acidovorax avenae with similarity values of 0.716, 0.705 and 0.723, respectively. Sequences analysis showed that the three representative strains were more than 99% consistent with A.avenae subsp. avenae. Physiological and biochemical reactions such as Gram staining and carbon utilization of these isolates were consistent with those of control strain A.avenae ICMP3183, thus the pathogen was eventually identified as Acidovorax avenae. The MZ121101 strain was sensitive to 80% ethylicin EC, 30% ethylicin EC and 0.3% tetramycin AS, the EC50 of them was 389.31-939.94 μg/mL。The two fungicides could be used to control bacterial stripe of millet in the field.
keywords:millet  bacterial stripe  Acidovorax avenae  pathogen identification  bactericide  susceptibility
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