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内蒙古自治区向日葵小核盘菌的菌丝亲和组类型及其菌株的生物学特性、致病力和交配型
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引用本文:贾瑞芳,李敏,张键,云晓鹏,孟庆林,赵君.内蒙古自治区向日葵小核盘菌的菌丝亲和组类型及其菌株的生物学特性、致病力和交配型.植物保护学报,2019,46(6):1222-1232
DOI:10.13802/j.cnki.zwbhxb.2019.2018160
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作者单位E-mail
贾瑞芳 内蒙古农业大学园艺与植物保护学院, 呼和浩特 010018  
李敏 内蒙古农业大学园艺与植物保护学院, 呼和浩特 010018  
张键 内蒙古农业大学园艺与植物保护学院, 呼和浩特 010018  
云晓鹏 内蒙古农牧业科学院植物保护研究所, 呼和浩特 010031  
孟庆林 黑龙江省农业科学院植物保护研究所, 哈尔滨 150086  
赵君 内蒙古农业大学园艺与植物保护学院, 呼和浩特 010018 zhaojun02@hotmail.com 
中文摘要:为明确内蒙古自治区阴山北麓地区向日葵小核盘菌Sclerotinia minor的遗传变异,对自内蒙古自治区乌兰察布市、包头市和呼和浩特市向日葵上分离纯化的110株向日葵小核盘菌菌株进行菌丝亲和组(mycelium compatibility group,MCG)划分,并对5个主要MCG组间和组内各菌株的生物学特性、致病力和交配型进行测定。结果表明,供试的110株菌株被划分为14个亲合组,其中MCG1为主要类型,包含32株菌株,占总菌株的29.1%;MCG2包含来自7个地点的25株菌株;5个MCG仅包含1株菌株;在这14个MCG中,MCG1~MCG5包含92株菌株,占总菌株数的83.6%。MCG1~MCG5组间各菌株在菌落直径和草酸分泌量上存在差异,但在菌核形成量、多聚半乳糖醛酸酶活性和致病力上无显著差异;而MCG1~MCG5组内各菌株在菌落直径、草酸分泌量、菌核形成量、多聚半乳糖醛酸酶活性和致病力上均有一定差异。在MCG1~MCG5各菌株的交配型中,除MCG2中菌株的负反转型与正反转型比例接近1:1外,其它4个MCG中菌株的负反转型与正反转型比例均偏离1:1,表明内蒙古自治区向日葵小核盘菌具有较高的遗传变异程度。
中文关键词:向日葵  小核盘菌  菌丝亲和组  致病力  交配型
 
Mycelium compatibility group of sunflower Sclerotinia minor and its biological characteristics, pathogenicity and mating type in Inner Mongolia Autonomous Region
Author NameAffiliationE-mail
Jia Ruifang College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia Autonomous Region, China  
Li Min College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia Autonomous Region, China  
Zhang Jian College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia Autonomous Region, China  
Yun Xiaopeng Institute of Plant Protection, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Huhhot 010031, Inner Mongolia Autonomous Region, China  
Meng Qinglin Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang Province, China  
Zhao Jun College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia Autonomous Region, China zhaojun02@hotmail.com 
Abstract:In order to determine genetic variation of sunflower Sclerotinia minor in the north foot of Yinshan Mountain, Inner Mongolia Autonomous Region, 110 S. minor strains were collected, isolated and purified, from Wulanchabu, Baotou and Hohhot cities, and were tested for mycelium compatibility group (MCG). The biological characteristics, pathogenicity and mating type of the five major MCGs were determined. The results showed that 110 strains were divided into 14 MCGs, of which MCG1 was the main type, including 32 strains, 29.1% of the total number of strains; MCG2 contained 25 strains which collected from seven locations; five MCGs contain only one strain. Among 14 MCGs, MCG1-MCG5 contained 92 strains, accounting for 83.6% of the total number of tested strains. There were differences in colony diameter and oxalic acid secretion between the MCG1-MCG5, but there was no significant difference in the number of sclerotia formation, virulence and polygalacturonase enzyme activity. However, each strain in MCG1-MCG5 showed significant variation on above parameters. The results of the mating type assay in MCG1-MCG5 indicated that the results of mating type ratio of negative inversion and positive inversion in other four MCGs showed the deviation from 1:1, except in MCG2, which the ratio was close to 1:1, indicating that S. minor had a high degree of genetic variation.
keywords:sunflower  Sclerotinia minor  mycelium compatibility group  pathogenicity  mating type
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