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棉花内生真菌CEF-373菌株对棉花黄萎病的防效及其作用机理
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引用本文:赵沛,冯自力,师勇强,赵丽红,胡小平,朱荷琴.棉花内生真菌CEF-373菌株对棉花黄萎病的防效及其作用机理.植物保护学报,2019,46(6):1203-1213
DOI:10.13802/j.cnki.zwbhxb.2019.2019008
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作者单位E-mail
赵沛 西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西 杨凌 712100
郑州大学, 郑州科研基地, 棉花生物学国家重点实验室, 郑州 450001 
 
冯自力 中国农业科学院棉花研究所, 棉花生物学国家重点实验室, 河南 安阳 455000  
师勇强 中国农业科学院棉花研究所, 棉花生物学国家重点实验室, 河南 安阳 455000  
赵丽红 中国农业科学院棉花研究所, 棉花生物学国家重点实验室, 河南 安阳 455000  
胡小平 西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西 杨凌 712100 heqinanyang@sohu.com 
朱荷琴 郑州大学, 郑州科研基地, 棉花生物学国家重点实验室, 郑州 450001
中国农业科学院棉花研究所, 棉花生物学国家重点实验室, 河南 安阳 455000 
xphu@nwsuaf.edu.cn 
中文摘要:为明确棉花内生真菌CEF-373菌株对棉花黄萎病的防效及其作用机理,利用圆盘滤膜法和平板对扣培养法测定菌株CEF-373对大丽轮枝菌Verticillium dahliae菌丝生长的抑制作用,测定其对棉花黄萎病的温室和田间防效,并通过活性氧含量及防御基因表达情况来分析其作用机理。结果表明,菌株CEF-373的挥发性代谢产物和非挥发性代谢产物对大丽轮枝菌菌丝的生长均有显著抑制作用,抑制率最高分别可达37.75%和100.00%。用1×107 CFU/mL的菌株CEF-373分生孢子悬浮液灌根后,对棉花黄萎病的温室防效可达71.12%,用质量比为3%的菌株CEF-373固体菌剂拌土栽培后,对棉花黄萎病的温室防效可达62.74%,防治作用显著。菌株CEF-373的发酵液滴灌和微生物肥料处理40 d后,对棉花黄萎病的田间防效达到最大,分别为36.23%和27.71%,而在后期有所降低。菌株CEF-373可以诱导棉花叶片中细胞活性氧的爆发;且菌株CEF-373成功激活了苯丙氨酸解氨酶、过氧化物酶、多酚氧化酶、几丁质酶和病程相关蛋白基因PR10的表达,对大丽轮枝菌的侵染具有抵御作用。表明棉花内生真菌CEF-373菌株通过抑制大丽轮枝菌生长以及诱导寄主系统抗病性来有效防治棉花黄萎病,具有较好的生物防治应用前景。
中文关键词:棉花黄萎病  内生真菌  大丽轮枝菌  生物防治  诱导抗性
 
Biological control effect and mechanism of cotton endophytic fungus Fusarium solani CEF-373 against Verticillium wilt in Gossypium hirsutum
Author NameAffiliationE-mail
Zhao Pei State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China
State Key Laboratory of Cotton Biology, Zhengzhou Research Base, Zhengzhou University, Zhengzhou 450001, Henan Province, China 
 
Feng Zili State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China  
Shi Yongqiang State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China  
Zhao Lihong State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China  
Hu Xiaoping State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China heqinanyang@sohu.com 
Zhu Heqin State Key Laboratory of Cotton Biology, Zhengzhou Research Base, Zhengzhou University, Zhengzhou 450001, Henan Province, China
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China 
xphu@nwsuaf.edu.cn 
Abstract:In order to determine the control effect and mechanism of cotton endophytic fungus CEF-373 on Verticillium wilt, the inhibition rate of strain CEF-373 against Verticillium dahliae was assessed using the exudate filtering method and plate-to-plate culture method, and the control effect of Verticillium wilt on cotton was tested with greenhouse and field experiments. Meanwhile, the content of reactive oxygen species and the expression of defense genes were detected to analyze the mechanism of action. The results showed that the growth inhibition rates of volatile metabolites and non-volatile metabolites of strain CEF-373 were up to 37.75% and 100.00% respectively. After root irrigation with 1×107 CFU/mL strain CEF-373 conidia suspension, the greenhouse control efficiency on Verticillium wilt of cotton was up to 71.12%, and the solid bacterial strain CEF-373 with a mass ratio of 3% was mixed with soil and cultivated, the greenhouse control efficiency was up to 62.74%, which had a significant control effect on Verticillium wilt. The field control efficiency of strain CEF-373 on Verticillium wilt of cotton was maximized by drip irrigation and microbial fertilizer treatment for 40 days, which was 36.23% and 27.71% respectively, but decreased in the later stage. Strain CEF-373 could induce the outbreak of reactive oxygen species in cells of cotton leaves, and successfully activated the expression of phenylalanine aminolytic enzyme, peroxidase, polyphenol oxidase, chitinase and pathogenesis-related gene RP10, which had a protective effect against the infection of V. dahlia. The results indicated that strain CEF-373 of endophytic fungus in cotton could effectively control Verticillium wilt of cotton by inhibiting the growth of V. dahliae and inducing disease resistance of host, which has a good application prospect in biocontrol.
keywords:cotton Verticillium wilt  endophytic fungus  Verticillium dahliae  biological control  induced resistance
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