| 撕裂蜡孔菌对烟草黑胫病的防治效果及对烤烟生长的影响 |
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| 引用本文：隋宗明,袁玲.撕裂蜡孔菌对烟草黑胫病的防治效果及对烤烟生长的影响.植物保护学报,2019,46(5):1138-1146 |
| DOI：10.13802/j.cnki.zwbhxb.2019.2018072 |
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| 中文摘要:为探讨撕裂蜡孔菌Ceriporia lacerata菌株HG2011对烟草黑胫病的防治效果和对烤烟的促生作用，利用该菌株制备发酵液和固体菌剂，通过拮抗和盆栽试验研究其对烟草黑胫病病原菌烟草疫霉Phytophthora nicotianae生长的抑制作用、对黑胫病的防治效果及对烟苗生长和养分吸收的影响。结果表明：撕裂蜡孔菌菌株HG2011能显著抑制烟草疫霉生长；在带毒平板试验中，含20%发酵液的培养基对烟草疫霉菌丝生长的抑制率达54.50%；在平板对峙试验中，菌株HG2011能使烟草疫霉菌丝扭曲、畸形和空泡化，最终完全侵入并覆盖烟草疫霉菌落。在盆栽试验中，施用撕裂蜡孔菌固体菌剂可降低发病烟苗叶片中的丙二醛含量，并能维持正常的细胞膜透性，使烟苗黑胫病发病率比单独接种烟草疫霉处理显著降低了15.55%~37.77%，且预防效果71.16%优于治疗效果17.98%。在化肥配施高量菌剂处理中烟苗的株高、最大叶面积和生物量均最高，比单施化肥处理显著增加9.08%、36.28%和11.84%；烟苗氮、磷、钾的吸收量比单施化肥处理显著增加11.29%、38.73%和12.79%。表明撕裂蜡孔菌菌株HG2011能有效防治烟草黑胫病，刺激烟苗生长、促进养分吸收，表现出良好的防病促生效应。 |
| 中文关键词:撕裂蜡孔菌 烤烟 黑胫病 养分 生长 |
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| Effects of Ceriporia lacerata on the control of black shank and growth promotion of flue-cured tobacco |
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| Abstract:In order to investigate the control effect of Ceriporia lacerata on tobacco black shank caused by Phytophthora nicotianae and its effect on the growth of flue-cured tobacco, a new strain HG2011 of C. lacerata was incubated to prepare fermentation broth and solid agent. Then antagonistic and pot tests were carried out to study its inhibitory effect on P. nicotianae, control efficiency against black shank, and influence on nutrient absorption and growth of tobacco seedlings. The results showed that the hyphal growth of P. nicotianae was obviously suppressed by the biocontrol fungus. In the plate antagonism test, the growth inhibition rate of P. nicotianae on the medium containing 20% fermentation broth reached 54.50%. In the dual culture test, C. lacerata HG2011 was able to intrude into P. nicotianae colonies and make pathogen hyphae distorted, vacuolated and deformed. In pot experiments, application of C. lacerata solid agent significantly decreased the concentration of malondialdehyde in leaves, and maintained normal permeability of leaf cell membrane compared with P. nicotianae inoculation. The black shank incidence was significantly decreased by 15.55%-37.77% compared with that in inoculation of P. nicotianae alone. The control efficacy was higher in preventative treatment (71.16%) than curative treatment (17.98%). After application of solid agent in combination with chemical fertilizers, plant height, maximum leaf area, and seedling biomass were significantly increased by 9.08%, 36.28% and 11.84%, respectively, compared with sole chemical fertilizer. The application of C. lacerata solid agent also significantly increased plant nitrogen uptake by 11.29%, phosphorus by 38.73%, and potassium by 12.79%. Therefore, C. lacerata HG2011 was able to effectively control tobacco black shank and improve growth and nutrient absorption of tobacco seedlings, showing a good application potential. |
| keywords:Ceriporia lacerata flue-cured tobacco black shank nutrition growth |
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