| 云南省荞麦叶枯病病原菌鉴定及其生物学特性 |
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| 引用本文：卢文洁,罗志明,李春花,尹桂芳,王艳青,王莉花.云南省荞麦叶枯病病原菌鉴定及其生物学特性.植物保护学报,2019,46(5):1065-1072 |
| DOI：10.13802/j.cnki.zwbhxb.2019.2018167 |
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| 作者 | 单位 | E-mail | | 卢文洁 | 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650205 | | | 罗志明 | 云南省农业科学院甘蔗研究所, 开远 616699 | | | 李春花 | 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650205 | | | 尹桂芳 | 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650205 | | | 王艳青 | 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650205 | | | 王莉花 | 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650205 | wanglihua70@hotmail.com |
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| 中文摘要:为明确云南省荞麦叶枯病病原菌种类，采用常规组织分离法获得病原菌菌株LW2015.3，通过形态学特征及分子生物学技术对菌株LW2015.3进行鉴定，并研究其生物学特性。结果表明，荞麦叶枯病病原菌的分生孢子呈倒棍棒状或倒梨状，褐色，具3~8个横隔膜，0~4个纵斜隔膜，大小为16.5~45.0 μm×5.0~13.5 μm，厚垣孢子呈球形，直径为6.0~12.0 μm；该菌株ITS序列系统发育进化分析结果表明，菌株LW2015.3与链格孢Alternaria alternata（登录号：MG195995.1）的同源性为100%，结合形态学特征与分子鉴定结果确定云南省荞麦叶枯病病原菌为链格孢A.alternata（登录号：KT362732.1）。该病原菌菌丝生长适宜温度为20~30℃，25℃为最适温度；当pH为6~9时菌丝生长速率加快，pH 7最适菌丝生长；PDA培养基和PSA培养基最适菌丝生长；该病原菌对以麦芽糖为碳源和以硝酸钠为氮源时的利用率最高；菌丝的致死温度为50℃，10 min；不同光照条件对菌丝生长的影响有显著差异，连续光照最有利于菌丝生长。 |
| 中文关键词:荞麦 叶枯病 病原菌鉴定 链格孢 生物学特性 |
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| Identification and biological characteristics of the pathogen causing leaf blight of Fagopyrum esculentum in Yunnan Province |
| Author Name | Affiliation | E-mail | | Lu Wenjie | Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Biotechnology and Genetic Resources Institute
Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China | | | Luo Zhiming | Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 616699, Yunnan Province, China | | | Li Chunhua | Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Biotechnology and Genetic Resources Institute
Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China | | | Yin Guifang | Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Biotechnology and Genetic Resources Institute
Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China | | | Wang Yanqing | Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Biotechnology and Genetic Resources Institute
Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China | | | Wang Lihua | Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Biotechnology and Genetic Resources Institute
Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China | wanglihua70@hotmail.com |
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| Abstract:To clarify the pathogen causing leaf blight of Fagopyrum esculentum, the strain LW2015.3 was obtained with the method of tissue isolation and identified based on morphological characteristics, molecular biological technology, and the biological characteristics of strain were also determined. The results showed that the fungal conidia were obclavate or obpyriform, brown, with three to eight transversely septate and zero to four longitudinally septate, 16.5-45.0 μm×5.0-13.5 μm in diameter. The chlamydospores were spherical, 6.0-12.0 μm in diameter. The ITS sequence of the strain LW2015.3 showed 100% homology with that of Alternaria alternata(MG195995.1). The pathogen was finally identified as A. alternata(KT362732.1). The mycelia could growth at 20-30℃, and 25℃ was the optimum temperature. The growth rate of mycelial increased rapidly at pH 6-9, and the optimum pH was 7. The optimum media for mycelial growth were PDA and PSA. The utilization rates of maltose and sodium nitrate were the highest for mycelial growth among all carbon and nitrogen sources, respectively. The fatal temperature for mycelial growth was 50℃ for 10 min. Significant differences were found in the mycelium growth under different light conditions and the continuous light was most favorable for mycelial growth. |
| keywords:Fagopyrum esculentum leaf blight pathogen identification Alternaria alternata biological characteristics |
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