| 桃蛀螟对青霉菌侵染苹果的产卵选择与行为趋向反应 |
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| 引用本文：石霞,艾莹婷,石芮竹,张民照,王海香,杜艳丽.桃蛀螟对青霉菌侵染苹果的产卵选择与行为趋向反应.植物保护学报,2019,46(2):434-442 |
| DOI：10.13802/j.cnki.zwbhxb.2019.2018127 |
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| 作者 | 单位 | E-mail | | 石霞 | 山西农业大学林学院, 太谷 030801
北京农学院植物科学技术学院, 植物生产国家级实验教学示范中心, 北京 102206 | | | 艾莹婷 | 北京农学院植物科学技术学院, 植物生产国家级实验教学示范中心, 北京 102206 | | | 石芮竹 | 北京农学院植物科学技术学院, 植物生产国家级实验教学示范中心, 北京 102206 | | | 张民照 | 北京农学院植物科学技术学院, 植物生产国家级实验教学示范中心, 北京 102206 | | | 王海香 | 山西农业大学林学院, 太谷 030801 | whx25@126.com | | 杜艳丽 | 北京农学院植物科学技术学院, 植物生产国家级实验教学示范中心, 北京 102206 | yanlidu@126.com |
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| 中文摘要:为探明桃蛀螟Conogethes punctiferalis偏好于在青霉菌Penicillium侵染的苹果上产卵的特点与机理，从苹果、玉米和柑橘上分离、纯化获得15株青霉菌，通过形态学和rDNA-ITS序列分析鉴定后，以健康苹果为对照，测试不同青霉菌菌株侵染的苹果对桃蛀螟产卵选择的影响，并利用四臂嗅觉仪测试桃蛀螟对青霉菌诱导的苹果挥发物的行为趋向反应。结果表明，15株青霉菌分别属于皮落青霉P.crustosum、橘青霉P.citrinum、苏门答腊青霉P.sumatrense和指状青霉P.digitatum的不同株型；桃蛀螟对其中的12株青霉菌侵染的苹果的产卵选择率均极显著高于健康苹果，尤其对皮落青霉CO5菌株、苏门答腊青霉OR3菌株和指状青霉OR5菌株侵染的苹果的产卵选择率最高，分别达到67.73%、61.50%和68.39%；桃蛀螟对感染皮落青霉CO5菌株和指状青霉OR5菌株的苹果挥发物的选择率分别为31.37%和29.19%，均显著高于对感染橘青霉OR1菌株和苏门答腊青霉OR3菌株的苹果挥发物的选择率18.14%和21.31%，且前二者之间、后二者之间均无显著差异。表明青霉菌可通过影响寄主植物挥发物释放，从而影响桃蛀螟对寄主植物的产卵选择和行为趋向反应。 |
| 中文关键词:桃蛀螟 苹果 青霉菌 产卵选择 行为趋向反应 |
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| Oviposition selection and behavioral tendency of yellow peach moth Conogethes punctiferalis (Guenée) to Penicillium-inoculated apples |
| Author Name | Affiliation | E-mail | | Shi Xia | College of Forestry, Agricultural University of Shanxi, Taigu 030801, Shanxi Province, China
National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China | | | Ai Yingting | National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China | | | Shi Ruizhu | National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China | | | Zhang Minzhao | National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China | | | Wang Haixiang | College of Forestry, Agricultural University of Shanxi, Taigu 030801, Shanxi Province, China | whx25@126.com | | Du Yanli | National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China | yanlidu@126.com |
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| Abstract:To illustrate the characteristics and mechanisms of oviposition preference of yellow peach moth Conogethes punctiferalis (Guenée) for Penicillium-inoculated apples, 15 Penicillium strains were isolated from apples, corns and oranges, and identified by morphological and molecular characters (sequences of rDNA-ITS). The attractiveness of apples infected by each Penicillium strain to C. punctiferalis was tested using oviposition selection, with healthy apples as control, and the behavioral tendency of C. punctiferalis to volatiles emitted from the apples infected by different Penicillium species was tested using four-arm olfactometer. The results showed that the 15 Penicillium strains belonged to P. crustosum, P. citrinum, P. sumatrense and P. digitatum, respectively. The oviposition selection rates of C. punctiferalis on the apples infected by 12 of the 15 Penicillium strains were significantly higher than those on healthy apples. Especially, the oviposition selection rates on the apples infected by the strains of P. crustosum CO5, P. sumatrense OR3 and P. digitatum OR5 reached 67.73%, 61.50% and 68.39%, respectively. The results of the behavioral tendency test showed that the selection rates of C. punctiferalis for P. crustosum CO5- (31.37%) and P. digitatum OR5-infected (29.19%) apples were both significantly higher than those for P. citrinum OR1- (18.14%) and P. sumatrense OR3-infected (21.31%) apples. However, the selection rates between P. crustosum- and P. digitatum-infected apples, as well as between P. citrinum- and P. sumatrense-infected apples, were not significantly different. It was suggested that Penicillium fungi could exert influence on the oviposition selection and behavioral tendency of C. punctiferalis to host plants, via affecting the emission of host plant volatiles. |
| keywords:Conogethes punctiferalis apple Penicillium oviposition selection behavior tendency |
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