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我国不同麦区小麦镰刀菌毒素检测及毒素污染分析

引用本文：马玉彤,刘太国,文才艺,刘博,高利,陈万权.我国不同麦区小麦镰刀菌毒素检测及毒素污染分析.植物保护学报,2019,46(2):266-273

DOI：10.13802/j.cnki.zwbhxb.2019.2018013

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作者	单位	E-mail
马玉彤	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 农业部农产品质量安全生物性危害因子(植物源)控制重点实验室, 北京 100193
刘太国	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 农业部农产品质量安全生物性危害因子(植物源)控制重点实验室, 北京 100193	tgliu@ippcaas.cn
文才艺	河南农业大学植物保护学院, 郑州 450002
刘博	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 农业部农产品质量安全生物性危害因子(植物源)控制重点实验室, 北京 100193
高利	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 农业部农产品质量安全生物性危害因子(植物源)控制重点实验室, 北京 100193
陈万权	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 农业部农产品质量安全生物性危害因子(植物源)控制重点实验室, 北京 100193	wqchen@ippcaas.cn

中文摘要:为明确镰刀菌毒素在我国不同小麦产区的污染与分布，利用超高效液相色谱-串联质谱仪对全国214个小麦品种籽粒中的7种镰刀菌毒素含量进行了测定和比较分析。结果表明：7种镰刀菌毒素的平均回收率在95.02%~116.52%之间，相对标准差为6.32%~21.50%。所有测试小麦样品均被镰刀菌毒素污染，7种毒素中以雪腐镰刀菌烯醇（nivalenol，NIV）含量最高，即使在NIV含量最低的长江中下游冬麦区，平均含量也高达182.56 μg/kg，虽然目前并没有关于NIV的相关限量标准，但也应该予以足够重视；脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）含量较高，在8.07~337.23 μg/kg之间，低于国家限量标准1 000 μg/kg；T-2含量在4.05~78.05 μg/kg之间，低于现有国际限量标准100 μg/kg；玉米赤霉烯酮（zearalmone，ZEA）含量在5.30~12.50 μg/kg之间，低于国家限量标准60 μg/kg；其它3种毒素含量较低，均低于17.73 μg/kg。不同麦区间毒素比较发现，DON和3-乙酰脱氧雪腐镰刀菌烯醇的含量在长江中下游冬麦区最高，分别为43.09 μg/kg和6.39 μg/kg，且显著高于其它麦区；来自长江中下游冬麦组和黄淮冬麦区南片水地组的小麦品种籽粒中毒素T-2和HT-2的含量显著高于其它麦区；其余毒素在不同麦区间均无显著差异。

中文关键词:超高效液相色谱-串联质谱镰刀菌毒素脱氧雪腐镰刀菌烯醇雪腐镰刀菌烯醇

Determination and contamination of Fusarium mycotoxins in different wheat planting regions of China

Author Name	Affiliation	E-mail
Ma Yutong	Key Laboratory of Biological Hazard Factors(Plant Source) Control of Agricultural Products Quality and Safety, Ministry of Agriculture and Rural Affairs State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Liu Taiguo	Key Laboratory of Biological Hazard Factors(Plant Source) Control of Agricultural Products Quality and Safety, Ministry of Agriculture and Rural Affairs State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China	tgliu@ippcaas.cn
Wen Caiyi	Plant Protection College, Henan Agricultural University, Zhengzhou 450002, Henan Province, China
Liu Bo	Key Laboratory of Biological Hazard Factors(Plant Source) Control of Agricultural Products Quality and Safety, Ministry of Agriculture and Rural Affairs State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Gao Li	Key Laboratory of Biological Hazard Factors(Plant Source) Control of Agricultural Products Quality and Safety, Ministry of Agriculture and Rural Affairs State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Chen Wanquan	Key Laboratory of Biological Hazard Factors(Plant Source) Control of Agricultural Products Quality and Safety, Ministry of Agriculture and Rural Affairs State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China	wqchen@ippcaas.cn

Abstract:To define the contamination and distribution of mycotoxins caused by wheat Fusarium head blight in China, ultraperformance liquid chromatography-tandem mass spectrometer was used to determine and analyze seven toxins from 214 wheat cultivars. The results showed that the mean recovery rate of seven toxins was between 95.02%- 116.52% and the relative standard deviation was 6.32%- 21.50%. All wheat cultivars were contaminated. The content of nivalenol (NIV) was the highest, with a minimum content of 182.56 μg/kg in wheat in the middle and lower reaches of the Yangtze River. However, there was no national standards for controlling the content of NIV from wheat cultivars, which should be paid attention to. The volume of deoxynivalenol (DON) was in the range of 8.07-337.23 μg/kg, which was less than 1 000 μg/kg of the national standard. The content of T-2 was from 4.05 μg/kg to 78.05 μg/kg according with international standard under 100 μg/kg. The content of zearalmone was between 5.30-12.50 μg/kg, lower than the national standard under 60 μg/kg. The contents of 3-acetyl deoxynivalenol (3-ADON), 15-acetyl deoxynivalenol and HT-2 were all less than 17.73 μg/kg. The average contents of DON and 3-ADON were the highest in wheat in the middle and lower reaches of the Yangtze River (43.09 μg/kg and 6.39 μg/kg, respectively), significantly higher than those in other wheat planting areas. The average contents of T-2 and HT-2 in the wheat in the middle and lower reaches of the Yangtze River and the irrigated winter wheat in south of Huang-Huai Region were significantly higher than those in other nine wheat regions.

keywords:UPLC-MS/MS Fusarium mycotoxin deoxynivalenol (DON) nivalenol (NIV)

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