| 枯草芽胞杆菌HMB-20428与化学杀菌剂互作对葡萄霜霉病菌抑制作用和替代部分化学药剂减量用药应用 |
| 点此下载全文 |
| 引用本文：毕秋艳,韩秀英,马志强,赵建江,王文桥,贾海民.枯草芽胞杆菌HMB-20428与化学杀菌剂互作对葡萄霜霉病菌抑制作用和替代部分化学药剂减量用药应用.植物保护学报,2018,45(6):1396-1404 |
| DOI：10.13802/j.cnki.zwbhxb.2018.2017224 |
| 摘要点击次数: |
| 全文下载次数: |
| 作者 | 单位 | E-mail | | 毕秋艳 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | | | 韩秀英 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | xiuyinghan@163.com | | 马志强 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | mazhiqiang304@163.com | | 赵建江 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | | | 王文桥 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | | | 贾海民 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | |
|
|
| 中文摘要:为明确生防菌剂枯草芽胞杆菌Bacillus subtilis HMB-20428与化学杀菌剂互作对葡萄霜霉病菌Plasmopara viticola的抑制作用，采用生物测定与田间药效试验研究其最佳用药时期，筛选最佳生化互作组合，结合空间竞争能力和诱导抗病性生理测定试验了解互作增效机制，并建立葡萄霜霉病的综合防控技术。结果显示，枯草芽胞杆菌HMB-20428预防葡萄霜霉病最佳时期为发病前1个月，连续喷雾用药3~4次，间隔期10~15 d。研发出协同增效生化互作组合生防菌剂HMB-20428+嘧菌酯，及其与硅氧烷化合物增效组合，防效分别为91.06%~98.92%和87.78%~92.04%。枯草芽胞杆菌HMB-20428与嘧菌酯互作可增强枯草芽胞杆菌HMB-20428定殖能力和植株抗病作用，且定殖能力和抗病机制作用的增强时间基本一致。以生防菌剂HMB-20428替代部分化学药剂减量用药流程的防效为91.40%。表明枯草芽胞杆菌HMB-20428与嘧菌酯互作可达到减少化学药剂用量的目的。 |
| 中文关键词:葡萄霜霉病 枯草芽胞杆菌 互作机制 化学减药流程 综合防控体系 |
| |
| Inhibitory effects of Bacillus subtilis HMB-20428 interacted with chemical fungicides and decrement of chemical fungicides on oomycete pathogen Plasmopara viticola |
| Author Name | Affiliation | E-mail | | Bi Qiuyan | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Integrated Pest Management Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | | | Han Xiuying | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Integrated Pest Management Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | xiuyinghan@163.com | | Ma Zhiqiang | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Integrated Pest Management Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | mazhiqiang304@163.com | | Zhao Jianjiang | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Integrated Pest Management Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | | | Wang Wenqiao | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Integrated Pest Management Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | | | Jia Haimin | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Integrated Pest Management Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | |
|
|
| Abstract:To clarify the inhibitory effect of Bacillus subtilis HMB-20428 interacted with chemical fungicides on oomycete pathogen Plasmopara viticola, the optimal treatment period and the best biochemical interaction combination were investigated by bioassay and field efficacy trials; the synergisticy mechanism was studied by mesuring the space competition ability and induced resistance, and the integrated prevention and control system for grape downy mildew was established. The results showed that the best time for the prevention of grape downy mildew with B. subtilis HMB-20428 was one month before the disease occurrence. The times of continuous spraying times was three to four with the intervals of ten to 15 days. The new synergistic combinations of fungicide and biocontrol agent (B. subtilis HMB-20428+ azoxystrobin or plus 100% siloxane compounds) were found for controlling grape downy mildew, with a control effect of 91.06%-98.92% and 87.78%-92.04%, respectively. The space competition ability and resistance induced by B. subtilis HMB-20428 were increased when combined with azoxystrobin, with a basically synchronous enhancement effect. The effect of the integrated prevention and control system for grape downy mildew mainly based on biological agent reached 91.40%. The results indicated that the interaction between B. subtilis HMB-20428 and azoxystrobin could reduce the dosage of chemical fungicides. |
| keywords:grape downy mildew Bacillus subtilis interaction mechanism chemical decrement procedure integrated prevention and control system |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 8738946 版权所有：植物保护学报 京ICP备05006550号-2 主管单位：中国科协 主办单位：中国植物保护学会、中国农业大学 地址：北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话：010-62732528 电子邮件：zbxb@cau.edu.cn 技术支持： 北京勤云科技发展有限公司 |
