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禾谷镰刀菌CYP51A基因对五种三唑类杀菌剂敏感性的影响

引用本文：钱恒伟,迟梦宇,赵颖,赵彦翔,黄金光.禾谷镰刀菌CYP51A基因对五种三唑类杀菌剂敏感性的影响.植物保护学报,2018,45(6):1381-1388

DOI：10.13802/j.cnki.zwbhxb.2018.2017108

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作者	单位	E-mail
钱恒伟	青岛农业大学植物医学学院, 山东省植物病虫害综合防控重点实验室, 青岛 266109
迟梦宇	青岛农业大学植物医学学院, 山东省植物病虫害综合防控重点实验室, 青岛 266109
赵颖	青岛农业大学植物医学学院, 山东省植物病虫害综合防控重点实验室, 青岛 266109
赵彦翔	青岛农业大学植物医学学院, 山东省植物病虫害综合防控重点实验室, 青岛 266109
黄金光	青岛农业大学植物医学学院, 山东省植物病虫害综合防控重点实验室, 青岛 266109	jghuang@qau.edu.cn

中文摘要:为进一步明确14α-脱甲基酶编码基因CYP51A在禾谷镰刀菌Fusarium graminearum对三唑类杀菌剂敏感性中的作用，利用Split-marker方法敲除禾谷镰刀菌CYP51A基因并获得敲除CYP51A基因的禾谷镰刀菌ΔFgCYP51A菌株，比较该菌株和野生型菌株PH-1对5种三唑类杀菌剂的敏感性变化。结果表明，ΔFgCYP51A菌株对丙环唑、戊唑醇、烯唑醇、三唑醇和三唑酮的EC₅₀分别为0.024、0.047、0.148、0.154和0.474 mg/L。ΔFgCYP51A菌株对戊唑醇、三唑醇、三唑酮和丙环唑的敏感性均显著增加，而对烯唑醇的敏感性无明显变化。表明FgCYP51A基因与禾谷镰刀菌对三唑类杀菌剂的敏感性有关。

中文关键词:禾谷镰刀菌 CYP51A基因三唑类杀菌剂敏感性抑制中浓度

Effect of CYP51A gene of Fusarium graminearum on sensitivity to five triazole fungicides

Author Name	Affiliation	E-mail
Qian Hengwei	Key Laboratory of Integrated Crop Disease and Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China
Chi Mengyu	Key Laboratory of Integrated Crop Disease and Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China
Zhao Ying	Key Laboratory of Integrated Crop Disease and Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China
Zhao Yanxiang	Key Laboratory of Integrated Crop Disease and Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China
Huang Jinguang	Key Laboratory of Integrated Crop Disease and Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China	jghuang@qau.edu.cn

Abstract:To understand the function of 14 alpha-demethylation encoding gene CYP51A on the sensitivity to triazole fungicides, the Split-marker approach was used to generate the FgCYP51A gene deletion mutant, which was named as the ΔFgCYP51A strain, and the sensitivity was investigated and compared between ΔFgCYP51A strain and wide type strain PH-1. The results showed that the EC₅₀ of ΔFgCYP51A strain to propiconazole, tebuconazole, diniconazole, triadimenol and triadimefon were 0.024, 0.047, 0.148, 0.154 and 0.474 mg/L, respectively. The sensitivity of ΔFgCYP51A strain to tebuconazole, triadimenol, triadimefon and propiconazole increased significantly compared to the PH-1 strain, but the diniconazole had no significant change. This research proved that the FgCYP51A gene was sensitive to triazole fungicide.

keywords:Fusarium graminearum CYP51A gene triazole fungicides sensitivity EC₅₀

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