| 河南省小麦赤霉病菌对咯菌腈的敏感性 |
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| 引用本文：徐建强,平忠良,马世闯,黄梦鸽,张祥辉,党威.河南省小麦赤霉病菌对咯菌腈的敏感性.植物保护学报,2018,45(6):1367-1373 |
| DOI：10.13802/j.cnki.zwbhxb.2018.2017119 |
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| 中文摘要:为明确河南省小麦赤霉病菌Fusarium graminearum对咯菌腈的敏感性，采用菌丝生长速率法测定了咯菌腈对从该省11个地市分离的95株菌株的毒力，通过方差分析法及聚类分析法对测定结果进行了分析，并研究了咯菌腈与多菌灵、戊唑醇对病菌毒力的相关性。结果显示：咯菌腈对小麦赤霉病菌菌丝生长的最低抑制浓度为0.1 μg/mL；咯菌腈对供试95株菌株的EC50范围在0.003~0.088 μg/mL之间，平均EC50为0.011 μg/mL；敏感性频率分布图显示，病菌群体中存在着对咯菌腈敏感性较低的亚群体，但67.4%供试菌株敏感性频率呈正态分布，将此部分菌株的EC50平均值0.007 μg/mL作为小麦赤霉病菌对咯菌腈的敏感性基线。方差分析及聚类结果均显示，同一县市内的菌株对咯菌腈EC50的最大值和最小值之比为1.1~8.3；除周口市沈丘县的菌株外，其余县市的菌株对咯菌腈敏感性差异不明显，咯菌腈EC50平均值变化范围在0.005~0.028 g/mL之间，最大值是最小值的5.6倍；小麦赤霉病菌对咯菌腈的敏感性与其对多菌灵、戊唑醇的敏感性之间无明显相关性。表明河南省小麦赤霉病菌群体中尽管存在着敏感性较低的亚群体，但可通过药剂复配进行防控。 |
| 中文关键词:河南省 小麦赤霉病菌 咯菌腈 菌丝生长 敏感性基线 |
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| Sensitivity of the isolates of Fusarium graminearum to fludioxonil in Henan Province |
| Author Name | Affiliation | E-mail | | Xu Jianqiang | College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan Province, China | xujqhust@126.com | | Ping Zhongliang | College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan Province, China | | | Ma Shichuang | College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan Province, China | | | Huang Mengge | College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan Province, China | | | Zhang Xianghui | College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan Province, China | | | Dang Wei | College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan Province, China | |
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| Abstract:In order to detect the sensitivity of Fusarium graminearum from Henan Province to fludioxonil, 95 isolates of F. graminearum were collected from 11 counties in 2016, and their sensitivities to fludioxonil were determined by measuring the mycelial growth on the fungicide-amended media, then the methods of least-significant difference (LSD) and SPSS cluster were used. The sensitivities to carbendazim and tebuconazole were also determined to analyze the correlation efficient existed between fludioxonil and the two fungicides, carbendazim and tebuconazole. The results showed that the minimum inhibition concentration, which referred to the concentration that could inhibit the mycelial growth totally, was 0.1 μg/mL. The EC50 values of all isolates ranged from 0.003 to 0.088 μg/mL and the average EC50 value was 0.011 μg/mL. The results of the sensitive frequency analysis revealed that there was low sensitive subcolony to fludioxonil in the tested isolates. The mean EC50 value of 0.007 μg/mL for 67.4% of all isolates showed a normal distribution, which was treated as the sensitive baseline of F. graminearum to fludioxonil. The results both variance analysis by LSD test and hierarchical cluster analysis by SPSS showed that the isolates separated from the same region demonstrated different sensitivities, which implied that the ratio of maximum and minimum of EC50 values of isolates from the same region ranged from 1.1 to 8.3. However, there was no significant difference on the sensitivity to fludioxonil among the isolates from most of the regions except those from Shenqiu County, Zhoukou City, which implied that the average EC50 value of the isolates from all the regions ranged from 0.005 to 0.028 μg/mL and the ratio of maximum and minimum of average EC50 values was 5.6. The correlation efficient existed between fludioxonil and carbendazim, tebuconazole was very low, which implied that there was no correlation of sensitivity of F. graminearum to the arbendazim or tebuconazole. Though there existed low sensitive subcolony to fludioxonil in the field, fludioxonil could still be used to control Fusarium head blight in combination with carbendazim or tebuconazole. |
| keywords:Henan Province Fusarium head blight fludioxonil mycelial growth sensitive baseline |
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