| 水稻恶苗病病原菌鉴定及室内药剂毒力测定 |
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| 引用本文：陈宏州,杨红福,姚克兵,束兆林,周华飞,庄义庆.水稻恶苗病病原菌鉴定及室内药剂毒力测定.植物保护学报,2018,45(6):1356-1366 |
| DOI：10.13802/j.cnki.zwbhxb.2018.2018019 |
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| 中文摘要:为探明水稻恶苗病病原菌种类以及9种药剂对各类病原菌的室内毒力，基于形态学特征、致病性测定和TEF1-α序列分析对病原菌进行鉴定，并采用菌丝生长速率法分别测定多菌灵、咪鲜胺、氰烯菌酯、戊唑醇、丙硫菌唑、叶菌唑、咯菌腈、氟啶胺和噁霉灵对分离所得病原菌的室内毒力。结果表明，水稻恶苗病病原菌为藤仓赤霉复合种（Gibberella fujikuroi species complex，GFSC）内的藤仓镰孢菌Fusarium fujikuroi、层出镰孢菌F.proliferatum、拟轮枝镰孢菌F.verticillioides和F.andiyazi。多菌灵对层出镰孢菌、拟轮枝镰孢菌、F.andiyazi、敏感及抗性藤仓镰孢菌的EC50均值分别为0.310、0.387、0.310、0.680和2.152 μg/mL；咪鲜胺对上述镰孢菌的EC50均值分别为0.010、0.043、0.017、0.038和0.110 μg/mL；氰烯菌酯、戊唑醇、丙硫菌唑、叶菌唑、咯菌腈、氟啶胺和噁霉灵对分离所得4种病原菌藤仓镰孢菌、层出镰孢菌、拟轮枝镰孢菌和F.andiyazi的EC50均值分别为0.002~0.097、0.014~0.078、0.044~0.343、0.019~0.074、0.033~0.466、0.019~0.146和13.957~85.558 μg/mL。4种病原菌个体对不同药剂的敏感性存在显著差异，其中对氰烯菌酯、戊唑醇和叶菌唑整体较为敏感，而对噁霉灵敏感性最低，但不同病原菌对药剂的敏感性规律却基本一致。 |
| 中文关键词:水稻恶苗病 病原鉴定 TEF1-α 毒力测定 |
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| Identification of rice bakanae pathogens and indoor toxicity test of fungicides |
| Author Name | Affiliation | E-mail | | Chen Hongzhou | Zhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province, Jurong 212400, Jiangsu Province, China | | | Yang Hongfu | Zhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province, Jurong 212400, Jiangsu Province, China | | | Yao Kebing | Zhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province, Jurong 212400, Jiangsu Province, China | | | Shu Zhaolin | Zhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province, Jurong 212400, Jiangsu Province, China | | | Zhou Huafei | Zhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province, Jurong 212400, Jiangsu Province, China | | | Zhuang Yiqing | Zhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province, Jurong 212400, Jiangsu Province, China | yqzhuang@sina.com |
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| Abstract:The pathogen identification of rice bakanae disease (RBD) based on the morphological characteristics, pathogenicity determination, and TEF1-α sequences analysis were carried out, and the indoor toxicity of nine fungicides, carbendazim, prochloraz, phenamacril, tebuconazole, prothioconazole, metaconazole, fludioxonil, fluazinam and hymexazol, to various pathogens were detected by using mycelium growth rate method, respectively. The results showed that the pathogens of RBD were identified as four species of the Gibberella fujikuroi species complex (GFSC):Fusarium fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi, respectively. According to the indoor tests, the EC50 mean values of carbendazim to F. proliferatum, F. verticillioides, F. andiyazi, as well as the sensitive and resistant F. fujikuroi were 0.310, 0.387, 0.310, 0.680 and 2.152 μg/mL, respectively, while the EC50 mean values of prochloraz were 0.010, 0.043, 0.017, 0.038 and 0.110 μg/mL, respectively. The EC50 values of phenamacril, tebuconazole, prothioconazole, metaconazole, fludioxonil, fluazinam and hymexazol to four pathogens, F. fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi, were 0.002-0.097, 0.014-0.078, 0.044-0.343, 0.019-0.074, 0.033-0.466, 0.019-0.146 and 13.957-85.558 μg/mL, respectively. There were significant differences on the sensitivity of any four pathogens to different fungicides. Overall, the pathogens were more sensitive to phenamacril, tebuconazole and metaconazole, with the lowest sensitivity to hymexazol. However, the sensitivity of different pathogens to each fungicide was basically the same. |
| keywords:rice bakanae disease pathogen identification translation elongation factor 1-α toxicity test |
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