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陕西省苹果树腐烂病菌基因多态性的ISSR分析

引用本文：臧睿,黄丽丽,李正力,高小宁,康振生.陕西省苹果树腐烂病菌基因多态性的ISSR分析.植物保护学报,2012,39(1):51-57

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作者	单位	E-mail
臧睿	西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100
黄丽丽	西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100	huanglili@nwsuaf.edu.cn
李正力	西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100
高小宁	西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100
康振生	西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100

中文摘要:为了从分子水平上揭示苹果树腐烂病菌的群体遗传多样性,采用正交设计对ISSR-PCR体系进行了4因素3水平的筛选,并从47条ISSR引物中筛选出11条多态性较好的引物。对供试的87个分离株进行扩增的结果显示,11条引物在129个位点扩增出稳定的条带,其中多态性位点119个,多态性位点率为92.25%。POPGENE分析显示,病菌种群的遗传多样性和基因多态性丰富,群体间的遗传分化系数(Gst)为0.109,群体内为0.891,群体内多样性大于群体间多样性。两个地理种群间的居群每代迁移数(Nm)为2.046,两者之间存在广泛的基因交流。在遗传相似系数为0.88时,可将21个自然种群划分为9个不同的类群,表明陕西省苹果树腐烂病菌的各个自然种群之间的遗传亲缘关系与其地理来源之间无明显的相关性。

中文关键词:苹果树腐烂病 ISSR-PCR扩增正交设计遗传多样性

Polymorphism analysis of Valsa mali isolated from Shaanxi Province using ISSR markers

Author Name	Affiliation	E-mail
Zang Rui	State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Huang Lili	State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China	huanglili@nwsuaf.edu.cn
Li Zhengli	State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Gao Xiaoning	State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Kang Zhensheng	State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China

Abstract:In order to detect the population genetic diversity of apple valsa canker pathogen (Valsa mali) at the molecular level, the orthogonal designed experiments were employed to screen the optimal inter-simple sequence repeat (ISSR) amplification protocol at three levels of four factors. Eleven ISSR primers which can amplify more polymorphic loci were screened from 47 ISSR primers. Eighty seven isolates of V.mali isolated from Shaanxi Province were studied for the genetic diversity. The results showed that 129 loci were detected of which 119 loci were polymorphic, the polymorphic loci percentage was 92.25%. The POPGENE analysis results showed that the genetic diversity of V.mali groups in Shaanxi was abundant, the coefficient of population genetic differentiation (Gst) among geographical groups was 0.109, while it reached 0.891 within the populations. The genetic diversity within the populations was much more abundant than among the groups. The number of migrants per generation between two geographical groups was 2.046, which indicated that genetic information exchange was frequent between the two groups. The dendrogram based on ISSR markers revealed that the 21 natural populations were clustered into 9 clusters at the threshold of genetic similar coefficient 0.88. There was no significant correlation between the genetic relationships and their geographical originals.

keywords:apple valsa canker ISSR-PCR amplification orthogonal design genetic diversity

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